beta-globin DNA in maternal plasma as a molecular marker of pre-eclampsia

Prenatal Diagnosis
Akihiko SekizawaTakashi Okai

Abstract

Levels of cell-free foetal DNA in maternal plasma are higher in the presence of clinical features of pre-eclampsia (PE). However, currently, this method is informative only in women bearing a male foetus, by amplification of Y-specific sequences. In the present study, we overcame this limitation by examining quantitative distribution of beta-globin, a foetal gender-independent DNA marker. We quantified beta-globin concentrations in the plasma of 207 pregnant women: control group, 164 subjects; affected group, 43 women affected by PE (n = 43). beta-globin concentrations were converted into multiples of the median of the controls (MoM), in order to assess the possible different distribution of beta-globin MoM in cases and controls. Adjusted MoM values were as follows: controls, 1.00 +/- 0.71; affected group 4.03 +/- 3.77 (p-value < 0.001). Among the PE affected cases, MoM beta-globin values of cases with foetal growth restriction (FGR) were almost twice as great as those cases without FGR (p-value = 0.003). beta-globin levels are higher in the plasma of pregnant women with PE, especially in those cases complicated with FGR, and do not depend on foetal gender. Such a molecular marker can potentially be used in evaluating the patho...Continue Reading

Citations

Jul 16, 2009·Reproductive Biology and Endocrinology : RB&E·Simon GrillOlav Lapaire
Jun 3, 2015·BMC Research Notes·Akhmad Yogi PramatirtaDwi Davidson Rihibiha
Mar 4, 2006·Expert Review of Molecular Diagnostics·Samuil R Umansky, L David Tomei
Oct 30, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Warunee TungwiwatNattaya Sae-Ung
May 24, 2014·Prenatal Diagnosis·Angela MartinAmber Samuel
Nov 16, 2007·The Journal of Obstetrics and Gynaecology Research·Akihiko SekizawaTakashi Okai
Jul 21, 2005·Prenatal Diagnosis·Keiko KoideTakashi Okai
Apr 4, 2020·Journal of Perinatal Medicine·Duygu AdiyamanAtalay Ekin
Jul 30, 2018·Molecular Diagnosis & Therapy·Danila MoranoAntonio Farina
Apr 14, 2005·Prenatal Diagnosis
Oct 27, 2021·Expert Review of Molecular Diagnostics·Ana C Palei

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