beta-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis.

Microbiology
R C MontijnFrans M Klis

Abstract

Wild-type cells of the pathogenic black yeast Exophiala (Wangiella) dermatitidis grown in a low-pH ascorbate medium became less melanized and less resistant to Zymolyase. This was accompanied by increased staining with fluorescently labelled concanavalin A. The sugar composition of wild-type and mutant cell walls was, except for the presence of galactose, similar to that of Saccharomyces cerevisiae. Digestion of mutant cell walls with laminarinase released galactomannoproteins. In addition, the released cell wall proteins contained glucose and reacted with affinity-purified 1,6-beta-glucan antiserum, indicating that they are linked to 1,6-beta-glucan. It is proposed that 1,6-beta-glucosylated cell wall proteins generally occur among ascomycetes.

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Citations

May 18, 2005·Fungal Genetics and Biology : FG & B·Piet W J De GrootFrans M Klis
Jan 8, 1999·Biochimica Et Biophysica Acta·J C KapteynF M Klis
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·M LussierH Bussey
Feb 25, 2006·Yeast·Frans M KlisPiet W J De Groot
Jan 1, 2020·Current Protein & Peptide Science·Laura C García-CarneroAlma K Tamez-Castrellón
Feb 15, 2000·Molecular Microbiology·S Shahinian, H Bussey
Jul 31, 1998·Journal of Bacteriology·P N Lipke, R Ovalle

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