PMID: 4895597Sep 1, 1969Paper

Beta granule formation in isolated islets of langerhans: a study by electron microscopic radioautography

The Journal of Cell Biology
S L HowellP E LACY

Abstract

The distribution of radioautographic grains over organelles within the beta cells of rat islets of Langerhans was investigated at various times after pulse labeling of the isolated islets with tritium-labeled amino acids. Ten minutes after the start of labeling most of the grains were situated over the endoplasmic reticulum and cytoplasm; by contrast, 60 min from the start of labeling the majority of the grains were associated with the beta granules. At 20, 30, and 45 minutes after pulse labeling the proportion of grains associated with the Golgi complex was increased two- to three-fold over the 10- or 60-minute values. The distribution of radioautographic grains over granules in the intact cells did not suggest that the electron-lucent type of secretory granules were precursors of the electron-opaque granules. Furthermore, studies of the pattern of grains over granules isolated by centrifugation 60 min after pulse labeling showed no preferential labeling of the electron-lucent type of granule. It is concluded that labeled amino acids are incorporated initially in the endoplasmic reticulum, and that the label subsequently appears in the beta granules. The Golgi complex participates either in the formation of the beta granule or...Continue Reading

References

Sep 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B M Kopriwa
Nov 1, 1967·Transactions of the New York Academy of Sciences·D F Steiner
May 1, 1968·Canadian Journal of Physiology and Pharmacology·J Logothetopoulos
Sep 1, 1960·The Journal of Clinical Investigation·A E RENOLDM C SHEPS
Dec 1, 1961·The American Journal of Medicine·P E LACY

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Citations

Jan 1, 1972·Naunyn-Schmiedeberg's Archives of Pharmacology·H WinklerH Hörtnagl
Oct 1, 1981·Clinical Biochemistry·A PermuttP Rotwein
Feb 1, 1976·Journal of Ultrastructure Research·S MoskalewskiU Friberg
Jan 1, 1976·Acta histochemica·L Orci
Oct 1, 1970·The New England Journal of Medicine·F MelaniD F Steiner
May 3, 1984·The New England Journal of Medicine·D C RobbinsA H Rubenstein
Nov 1, 1973·The Journal of Cell Biology·C R Hopkins, M G Farquhar
Oct 1, 1981·The Journal of Cell Biology·M M Salpeter, M G Farquhar
Sep 1, 1973·European Journal of Clinical Investigation·C CreutzfeldtW Creutzfeldt
Aug 1, 1971·Acta Pathologica Japonica·I NakayamaH Tsuchiyama
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Jul 1, 1970·The Journal of Clinical Investigation·T TaneseL Recant
Aug 1, 1975·Diabetologia·L Aerts, F A Assche
Feb 1, 1972·CRC Critical Reviews in Biochemistry·A E KitabchiS Yu
Aug 1, 1977·The Anatomical Record·B G SlavinS P Bessman
Dec 9, 1970·Biochemical and Biophysical Research Communications·W Kemmler, D F Steiner
May 19, 1988·Diabetes Research and Clinical Practice·A K Chatterjee, H Schatz
Nov 1, 1972·Clinics in Endocrinology and Metabolism·K W Taylor
Nov 1, 1972·Clinics in Endocrinology and Metabolism·N R Lazarus
Jan 1, 1972·Acta diabetologica latina·F Melani
Jan 1, 1975·CRC Critical Reviews in Toxicology·L J Fischer, D E Rickert

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