Beta-hydroxy-butyrate alters the extracellular content of S100B in astrocyte cultures

Brain Research Bulletin
Marina C LeiteCarlos-Alberto Gonçalves

Abstract

Astrocytes have a variety of roles in maintaining neural tissue physiology, including energetic support, uptake and metabolism of glutamate and secretion of neurotrophic factors. Glutamate toxicity has been implicated in neurodegenerative disorders associated with conditions related to energy failure, and to elevation of glutamate extracellular levels in brain. Glucose is the main energetic substrate for brain cells but, in some circumstances, the ketone bodies are used as a supplementary source and have been suggested to be neuroprotective agents against seizure disorders. Here, we investigate some possible biochemical changes in astrocyte cultures induced by beta-hydroxy-butyrate, the predominant blood ketone body. Its effect upon S100B secretion, astrocyte morphology and glutamate uptake was particularly investigated. S100B, a calcium-binding protein expressed and secreted by astrocytes, has neurotrophic activity and a possible role in epileptogenesis. Cell morphology was investigated by phase-contrast microscopy and immunocytochemistry for actin, GFAP and S100B. Our data show that beta-hydroxy-butyrate induces dramatic changes in astrocyte morphology and, independent of this, causes changes in the extracellular content of S...Continue Reading

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Citations

Apr 4, 2008·Journal of Neural Transmission·Kristian HoltkampMatthias Rothermundt
Jun 8, 2007·Cellular and Molecular Neurobiology·Lúcia Maria Vieira de AlmeidaCarlos-Alberto Gonçalves
Apr 19, 2008·Molecular and Cellular Biochemistry·André Quincozes-SantosCarmem Gottfried
Sep 15, 2010·Neurotoxicity Research·Iwona Chmiel-PerzyńskaEwa M Urbańska
Feb 24, 2006·Nutritional Neuroscience·M C SilvaC A Gonçalves
Oct 6, 2011·Journal of Neuroinflammation·Maria Cristina GuerraCarlos-Alberto Gonçalves
Nov 30, 2004·Neuroscience Research·Denize R ZieglerCarlos-Alberto Gonçalves
Nov 6, 2004·Brain Research·Regina MargisCarlos-Alberto Gonçalves
Jun 3, 2006·Archives of Medical Research·Cristina B O NettoCarlos-Alberto Gonçalves
Jun 4, 2013·Autonomic Neuroscience : Basic & Clinical·Chloe Stenkamp-StrahmOnesmo Balemba
Jul 12, 2017·Nutritional Neuroscience·Nathan M D'CunhaNenad Naumovski
Nov 6, 2018·Neurochemical Research·Adriana Fernanda K VizueteCarlos-Alberto Gonçalves
Apr 6, 2013·Life Sciences·Adriana Fernanda VizueteCarlos-Alberto Gonçalves
May 6, 2008·Clinical Biochemistry·Carlos-Alberto GonçalvesPatrícia Nardin

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