PMID: 7028919Nov 1, 1981Paper

beta-Hydroxybutyrate as a precursor to the acetyl moiety of acetylcholine

Journal of Neurochemistry
G H SterlingJ J O'Neill

Abstract

Rat brain cortex slices were incubated with 10 mM-glucose and trace amounts of [6-3H]glucose and [3-14C]beta-hydroxybutyrate. The effects of (-)-hydroxycitrate, an inhibitor of ATP-citrate lyase; methylmalonate, an inhibitor of beta-hydroxybutyrate dehydrogenase; and increasing concentrations of unlabeled acetoacetate were examined. The incorporation of label into lactate, citrate, malate, and acetylcholine (ACh) was measured and 3H:14C ratios calculated. Incorporation of [14C]beta-hydroxybutyrate into lactate was limited because of the low activity of gluconeogenic enzymes in brain, whereas incorporation of 14C label into Krebs cycle intermediates and ACh was higher than in previous experiments with [3H-,14C]-glucose. (-)-Hydroxycitrate (5.0 mM) reduced incorporation of [3H]glucose and [14C]beta-hydroxybutyrate into ACh. In contrast, slices incubated with methylmalonate (1 mM) showed a decrease in 14C incorporation without appreciably affecting glucose metabolism. The effects of high concentrations of methylmalonate were nonselective and yielded a generalized decrease in metabolism. Acetoacetate (1 mM) also produced a decreased 14C incorporation into ACh and its precursors. At 10 mM, acetoacetate reduced 3H and 14C incorporati...Continue Reading

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Citations

Apr 15, 1986·Biochimica Et Biophysica Acta·M ItoK Tomita
Jan 1, 1984·Progress in Biophysics and Molecular Biology·S Tucek
Jan 1, 1985·Journal of Neurochemistry·S Tucek
Jul 28, 2018·Frontiers in Cellular Neuroscience·Anna RonowskaAgnieszka Jankowska-Kulawy
Jan 5, 2002·Journal of Agricultural and Food Chemistry·B S JenaK K Sakariah
Aug 5, 2008·Neurochemistry International·Bjørnar HasselAndreas Lossius

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