beta-Lactamase activity in human pus

The Journal of Infectious Diseases
R E BryantD Hammond

Abstract

Pus was obtained from patients with polymicrobial intraabdominal abscesses or polymicrobial empyema. Physical and chemical characteristics of 12 specimens were examined, and bacterial isolates were enumerated. Pus supernatant of six specimens rapidly inactivated penicillin, cephalothin, and cefazolin. Carbenicillin and ticarcillin were similarly degraded by supernatant of certain pus specimens. Cefoxitin, chloramphenicol, and clindamycin were not appreciably inactivated by pus supernatant. Degradation of penicillin and cephalosporin congeners in pus was due to the presence of beta-lactamase, as shown by chemical interaction with nitrocefin, chromatography, and inhibition by the beta-lactamase inhibitor clavulanic acid. Pus supernatant containing beta-lactamase activity reduced the bactericidal activity of carbenicillin against Bacteroides fragilis in whole pus in an abscess model in vitro. Bactericidal activity of clindamycin or cefoxitin was not impaired in pus containing beta-lactamase.

Citations

Apr 1, 1987·European Journal of Clinical Microbiology·R E BryantE M Gardner
Feb 11, 2011·Current Infectious Disease Reports·Itzhak Brook
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·S GrinsteinO D Rotstein
Jul 15, 2005·Journal of Chromatography. a·Martina PetschErnst Kenndler
Sep 1, 1990·Respiratory Medicine·C I TimonM T Cafferkey
Aug 6, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Thomas A Heming, Akhil Bidani
Feb 21, 2006·Chemical Research in Toxicology·Vijaya M LakshmiTerry V Zenser
Sep 26, 2008·Nature·Reshma SinghPeter Cresswell
Mar 2, 2005·Diabetes Technology & Therapeutics·Christian PoulsenCarsten Worsøe
Feb 24, 2007·Diabetes Technology & Therapeutics·Christian PoulsenCarsten Worsøe
Jul 1, 1984·Antimicrobial Agents and Chemotherapy·N V JacobusM Barza
Jul 1, 1984·Antimicrobial Agents and Chemotherapy·R H EngC Cherubin
Jul 29, 2005·Antimicrobial Agents and Chemotherapy·D T A te DorsthorstJ W Mouton
Dec 17, 2009·BMC Infectious Diseases·Itzhak Brook
May 17, 2012·Biointerphases·Toru Yoshitomi, Yukio Nagasaki
Mar 7, 2014·The ISME Journal·Ana E Duran-PinedoJorge Frias-Lopez
Oct 4, 2012·FEMS Microbiology Reviews·Elizabeth Carmel Murphy, Inga-Maria Frick
Jun 1, 1991·Clinical Biochemistry·S GrinsteinO D Rotstein
May 1, 1986·The Journal of Pediatrics·J Levy
Sep 1, 1988·The Journal of Surgical Research·O D RotsteinD R Knighton
Apr 1, 1989·The Annals of Otology, Rhinology, and Laryngology·I Brook, P Yocum
Apr 8, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Iviana M TorresBrent Berwin
Nov 3, 2006·American Journal of Veterinary Research·Steven W MillsDouglas W Morck
Jul 26, 2006·Pharmacology·Claudia WagnerChristian Joukhadar
Sep 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo Akerström, Lars Björck
Jun 29, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mingfang LuRobert S Munford
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diego MartínezJorge Geffner
Apr 5, 2011·Molecular and Cellular Biochemistry·Neha Mittal, Sankar Nath Sanyal
Jan 30, 2019·Mediators of Inflammation·Fernando Erra DíazJorge Geffner
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mónica VermeulenJorge R Geffner
Oct 1, 1986·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·J E Jansen, A Bremmelgaard
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diego MartínezJorge Geffner
Feb 1, 1997·FEMS Immunology and Medical Microbiology·K B Abdul-MajidJ J Finlay-Jones

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Beta-lactamase Inhibitors

Beta-lactamase inhibitors are a class of antibiotics that inhibit beta-lactamases, a family of enzymes involved in bacterial resistance to beta-lactam antibiotics. Here is the latest research.

Beta-lactamase Inhibitors (ASM)

Beta-lactamase inhibitors are a class of antibiotics that inhibit beta-lactamases, a family of enzymes involved in bacterial resistance to beta-lactam antibiotics. Here is the latest research.