beta-MHC transgene expression in suspended and mechanically overloaded/suspended soleus muscle of transgenic mice

The American Journal of Physiology
J J McCarthyR W Tsika

Abstract

Non-weight-bearing (NWB) activity [space flight and hindlimb suspension (HS)] results in the loss of soleus muscle mass, a slow-to-fast fiber-type conversion, and decreased beta-myosin heavy chain (beta-MHC) protein and mRNA expression. To identify beta-MHC promoter sequences required for decreased beta-MHC expression in response to HS, we have modified an existing noninvasive hindlimb unweighting model to accommodate the use of (transgenic) mice. After 2 wk of HS, body and muscle (soleus > gastrocnemius > plantaris) weights were decreased as was the proportion of histochemically classified type I fibers in HS soleus muscle. Northern blot analysis revealed decreases in endogenous mRNA representing beta-MHC, slow myosin light chain 1 and 2, and cardiac/slow troponin C, whereas those representing skeletal troponin C, muscle creatine kinase, and glyceraldehyde-3-phosphate dehydrogenase increased. Protein extracts prepared from HS soleus (SS) muscle of mice harboring transgenes comprised of 5.6 or 0.6 kilobase of wild type (wt) mouse beta-MHC promoter (beta 5.6 wt, beta 0.6wt) and those carrying the simultaneous mutation (mut) of the MCAT, C-rich, and beta e3 subregions (beta 5.6mut3, beta 0.6mut3) revealed decreases in chloramphen...Continue Reading

Citations

Feb 8, 2002·American Journal of Physiology. Cell Physiology·K A HueyKenneth M Baldwin
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Richard W TsikaGretchen L Tsika
Apr 19, 2002·Journal of Applied Physiology·Manabu InobeShin'Ichi Takeda
Sep 2, 2003·Journal of Applied Physiology·Julian E Stelzer, Jeffrey J Widrick
Jul 6, 2020·Journal of Cell Science·Kristen K BjorkmanLeslie A Leinwand
Aug 5, 2000·Journal of Applied Physiology·R H FittsJ J Widrick
Mar 16, 2002·Journal of Applied Physiology·Emily R Morey-Holton, Ruth K Globus
Dec 2, 2004·Molecular and Cellular Biology·Gretchen TsikaRichard Tsika
May 18, 2006·Physiological Genomics·Heather L NicholsXuejun Wen
Nov 22, 2002·American Journal of Physiology. Cell Physiology·K A HueyK M Baldwin
Jul 29, 2003·Journal of Applied Physiology·B C HarrisonL A Leinwand
Aug 20, 2009·Physiological Genomics·John J McCarthyEsther E Dupont-Versteegden
Oct 14, 2004·Physiological Genomics·Christoph DäppMartin Flück
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J M GigerK M Baldwin
Dec 14, 2004·Journal of Applied Physiology·Julia M GigerKenneth M Baldwin
Aug 30, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Esther E Dupont-VersteegdenCharlotte A Peterson
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Patricia L Hallauer, Kenneth E M Hastings

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