Beta-nerve growth factor promotes neurogenesis and angiogenesis during the repair of bone defects

Neural Regeneration Research
Wei-Hui ChenJoo L Ong

Abstract

We previously showed that the repair of bone defects is regulated by neural and vascular signals. In the present study, we examined the effect of topically applied β-nerve growth factor (β-NGF) on neurogenesis and angiogenesis in critical-sized bone defects filled with collagen bone substitute. We created two symmetrical defects, 2.5 mm in diameter, on either side of the parietal bone of the skull, and filled them with bone substitute. Subcutaneously implanted osmotic pumps were used to infuse 10 μg β-NGF in PBS (β-NGF + PBS) into the right-hand side defect, and PBS into the left (control) defect, over the 7 days following surgery. Immunohistochemical staining and hematoxylin-eosin staining were carried out at 3, 7, 14, 21 and 28 days postoperatively. On day 7, expression of β III-tubulin was lower on the β-NGF + PBS side than on the control side, and that of neurofilament 160 was greater. On day 14, β III-tubulin and protein gene product 9.5 were greater on the β-NGF + PBS side than on the control side. Vascular endothelial growth factor expression was greater on the experimental side than the control side at 7 days, and vascular endothelial growth factor receptor 2 expression was elevated on days 14 and 21, but lower than con...Continue Reading

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Citations

Apr 8, 2017·Experimental & Molecular Medicine·Pan JinXingdong Zhang
May 24, 2018·Nanomedicine·Chen AijieShao Longquan
Dec 19, 2016·BioMed Research International·Xiao-Yi ZhouXian-Zhao Wei
Sep 16, 2020·International Journal of Molecular Sciences·Karin Wuertz-KozakPia M Wippert
Nov 22, 2020·Nature Reviews. Rheumatology·Barton L WiseNancy E Lane
Apr 16, 2021·Advanced Science·Qian-Qian WanKai Jiao
Oct 16, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lan ZhangHai Tang

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