Beta-tricalcium phosphate ceramic triggers fast and robust bone formation by human mesenchymal stem cells

Journal of Tissue Engineering and Regenerative Medicine
Rui C PereiraChiara Gentili

Abstract

Due to their osteoconductive and inductive properties, a variety of calcium phosphate (CaP) scaffolds are commonly used in orthopaedics as graft material to heal bone defects. In this study, we have used two CaP scaffolds with different hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) ratios (MBCP®; 60/40 and MBCP+ ®; 20/80) to investigate their intrinsic capacity to favour human bone marrow stem cells (hBMSCs) osteogenic differentiation capacity. We report that MBCP+ ® showed in in vitro culture model a higher rate of calcium ion release in comparison with MBCP®. In two defined coculture systems, the hBMSC seeded onto MBCP+ ® presented an increased amount of VEGF secretion, resulting in an enhanced endothelial cell proliferation and capillary formation compared with hBMSC seeded onto MBCP®. When both ceramics combined with hBMSC were implanted in a nude mouse model, we observed a faster osteogenic differentiation and enhancement mature bone deposition sustained by the presence of a vast host vasculature within the MBCP+ ® ceramics. Bone formation was observed in samples highly positive to the activation of calcium sensing receptor protein (CaSr) on the surface of seeded hBMSC that also shown higher BMP-2 protein expressi...Continue Reading

References

Feb 24, 2001·The New England Journal of Medicine·R QuartoM Marcacci
May 16, 2003·Nature·Henry M Kronenberg
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
May 1, 1960·The Journal of Bone and Joint Surgery. British Volume·J TRUETA, K LITTLE
Apr 10, 2004·Development·Elazar ZelzerBjorn R Olsen
Apr 21, 2004·Annals of Biomedical Engineering·Y LiuE B Hunziker
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·Guy DaculsiPierre Weiss
Feb 26, 2005·Journal of Cell Science·Aldebaran M Hofer
Sep 26, 2006·Tissue Engineering·Jeroen RouwkemaClemens A Van Blitterswijk
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dinithi PeirisR John MacLeod
Oct 12, 2007·Physiology·Aldebaran M Hofer, Konstantinos Lefkimmiatis
May 6, 2008·European Cells & Materials·J M Kanczler, R O C Oreffo
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ehsan JabbarzadehCato T Laurencin
Aug 16, 2008·The Journal of Biological Chemistry·Takuma MatsubaraToshiyuki Yoneda
Nov 14, 2008·Chemical Reviews·Racquel Zapanta LeGeros
Feb 5, 2009·Critical Reviews in Eukaryotic Gene Expression·Carrie S SoltanoffYi-Ping Li
May 22, 2009·Nature Materials·Elsie S PlaceMolly M Stevens
Nov 7, 2009·Cytokine & Growth Factor Reviews·Vicki Rosen
Jul 14, 2010·Acta Biomaterialia·Shahram GhanaatiC James Kirkpatrick
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Huipin YuanJoost D de Bruijn
Feb 2, 2011·Advanced Drug Delivery Reviews·C James KirkpatrickRonald E Unger
Mar 15, 2011·Advanced Drug Delivery Reviews·Esther C NovoselPetra J Kluger
May 24, 2011·European Cells & Materials·Ana M C BarradasPamela Habibovic
Jun 8, 2011·Trends in Biotechnology·Anna TampieriFederica Valentini
Jun 12, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ana M C BarradasJan de Boer
Mar 18, 2015·Journal of Materials Science. Materials in Medicine·Guy Daculsi
Jul 21, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Barbara CancianiSara Tavella

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