beta1 and beta2 integrins activate different signalling pathways in monocytes

The Biochemical Journal
Merit Reyes-ReyesC Rosales

Abstract

Integrin-mediated signals play an important but poorly understood role in regulating many leucocyte functions. In monocytes and macrophages, integrins of the beta2 subfamily are involved in cell-cell interactions that are important for migration of the cells through the endothelium and also for phagocytosis. On the other hand, in the same cells, beta1 integrin-mediated adhesion to extracellular matrix proteins results in a strong induction of immediate early genes that are important in inflammation. To investigate the signalling pathways from these two types of integrin in monocytic cells, THP-1 cells were selectively stimulated via beta1 or beta2 integrins by cross-linking each type of receptor with specific monoclonal antibodies or their natural ligands. The involvement of extracellular signal-regulated kinase (ERK), Syk and phosphoinositide 3-kinase (PI-3K) was then analysed. Nuclear factor kappaB (NF-kappaB) activation was also detected in THP-1 cells transiently transfected with an NF-kappaB-driven luciferase reporter gene. We found that binding of both types of integrin to their natural ligands activated ERK in a Syk- and PI-3K-dependent manner. Yet, cross-linking of integrins by anti-beta1 antibodies caused activation of...Continue Reading

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Citations

Jul 25, 2006·Cancer Immunology, Immunotherapy : CII·Nancy MoraCarlos Rosales
Jan 7, 2004·Biochemical and Biophysical Research Communications·Jae Youl ChoJee H Jung
Apr 10, 2004·Cancer Letters·Brett M HallLaura F Gibson
Dec 23, 2003·Immunology Letters·Alejandro Ortiz-Stern, Carlos Rosales
Jun 1, 2004·Inflammatory Bowel Diseases·Thomas MenzelWolfgang Scheppach
Jan 28, 2003·Cancer·Héctor Rincón-AranoCarlos Rosales
Sep 21, 2010·Bone·Jochen G SchneiderKatherine N Weilbaecher
Nov 19, 2003·Biochemical and Biophysical Research Communications·María Teresa de la FuenteAngeles Garcia-Pardo
Oct 17, 2014·Immunological Reviews·Spencer A Freeman, Sergio Grinstein
Jun 14, 2003·Biochemical and Biophysical Research Communications·Sergiy SukhanovPatrick Delafontaine
Dec 14, 2005·Biochemical and Biophysical Research Communications·Bjarne KrebsHans A Kretzschmar
Aug 23, 2005·Current Opinion in Cell Biology·Sharona Even-Ram, Kenneth M Yamada
Nov 17, 2005·Journal of Consulting and Clinical Psychology·Ricardo F Muñoz, Tamar Mendelson
Jan 21, 2004·The Journal of Biological Chemistry·Cindy M Sondag, Colin K Combs
Jul 12, 2017·BioMed Research International·Carlos Rosales, Eileen Uribe-Querol
Apr 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jan Herter, Alexander Zarbock

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