Beta1 integrins activate a MAPK signalling pathway in neural stem cells that contributes to their maintenance

Development
Lia Scotti CamposCharles ffrench-Constant

Abstract

The emerging evidence that stem cells develop in specialised niches highlights the potential role of environmental factors in their regulation. Here we examine the role of beta1 integrin/extracellular matrix interactions in neural stem cells. We find high levels of beta1 integrin expression in the stem-cell containing regions of the embryonic CNS, with associated expression of the laminin alpha2 chain. Expression levels of laminin alpha2 are reduced in the postnatal CNS, but a population of cells expressing high levels of beta1 remains. Using neurospheres - aggregate cultures, derived from single stem cells, that have a three-dimensional architecture that results in the localisation of the stem cell population around the edge of the sphere - we show directly that beta1 integrins are expressed at high levels on neural stem cells and can be used for their selection. MAPK, but not PI3K, signalling is required for neural stem cell maintenance, as assessed by neurosphere formation, and inhibition or genetic ablation of beta1 integrin using cre/lox technology reduces the level of MAPK activity. We conclude that integrins are therefore an important part of the signalling mechanisms that control neural stem cell behaviour in specific a...Continue Reading

References

Aug 1, 1995·Genes & Development·R Fässler, M Meyer
Jan 1, 1995·Experimental Brain Research·C N SvendsenS B Dunnett
Feb 27, 1999·Genes & Development·K A LawsonB L Hogan
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A J ZhuF M Watt
Feb 26, 2000·Science·F M Watt, B L Hogan
Jul 14, 2000·Molecular Endocrinology·Y YingG Q Zhao
Aug 1, 2000·Brain Research. Developmental Brain Research·R D LearishM Haak-Frendscho
Nov 1, 2000·Cell·J Schlessinger
Nov 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A SuzukiH Taniguchi
Jul 6, 2001·Cell·H M BlauJ M Weimann
Oct 3, 2001·Nature Biotechnology·C XuM K Carpenter
Nov 2, 2001·Nature·A SpradlingT Kai
Feb 28, 2002·Molecular and Cellular Neurosciences·Laurence DeckerAnne Baron-Van Evercooren
Apr 11, 2002·Nature Cell Biology·Kenneth M Yamada, Sharona Even-Ram
May 10, 2002·Genes & Development·Michel KalamaridesMarco Giovannini
Jan 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·J Peter Gergen, Eric F Wieschaus

❮ Previous
Next ❯

Citations

Aug 21, 2009·Cell and Tissue Research·Malgorzata BarczykDonald Gullberg
Nov 1, 2005·Cancer Metastasis Reviews·C Brakebusch, R Fässler
Sep 14, 2007·Journal of Molecular Histology·Barbara Murdoch, A Jane Roskams
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yi WangWei-dong Le
Jun 6, 2009·Journal of Molecular Neuroscience : MN·Matthew M HarperDonald S Sakaguchi
Jun 9, 2005·Progress in Retinal and Eye Research·Ani V DasIqbal Ahmad
Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hyun-Jai ChoHyo-Soo Kim
Jul 4, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Darryl R Davis, Duncan J Stewart
Jul 31, 2008·The Journal of Biological Chemistry·Susan C Kirkland, Huijun Ying
May 2, 2008·Stem Cells and Development·J MokrýD English
May 22, 2008·Tissue Engineering. Part a·Cristina Martínez-RamosJosé Miguel Soria
Jul 30, 2010·Tissue Engineering. Part a·Sarah E StabenfeldtMichelle C LaPlaca
Aug 10, 2011·Tissue Engineering. Part a·Rachael MooneyMelissa Mahoney
Apr 17, 2009·Tissue Engineering. Part C, Methods·Erin K PurcellDaryl R Kipke
Nov 25, 2005·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Sep 29, 2005·Neuroreport·Takahiko HamasakiMitsuo Ochi
Mar 4, 2006·Neuroreport·Bao-Feng MaAndy Peng Xiang
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·Claudia S BarrosUlrich Müller
Jun 3, 2009·Molecular Pharmacology·Manveen K GuptaDianne M Perez
Sep 24, 2008·BMC Developmental Biology·Balázs V VargaBalázs Herberth
Jul 25, 2008·BMC Neuroscience·Peter E HallCharles Ffrench-Constant
Aug 19, 2009·PLoS Biology·Karine LoulierCharles Ffrench-Constant
Mar 16, 2007·PloS One·Tamara GlaserOliver Brüstle
Jul 28, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ilias KazanisCharles ffrench-Constant
Apr 8, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ellen JorissenPaul Saftig
Jun 19, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Randor RadakovitsUlrich Müller
May 31, 2011·Frontiers in Bioscience (Scholar Edition)·Marnie Preston, Larry S Sherman
Aug 6, 2009·Environmental Health Perspectives·Michaela MoorsEllen Fritsche
Oct 2, 2009·Future Oncology·Patricia M McGowanAnn F Chambers
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Masanori SakaguchiHideyuki Okano
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konrad UrbanekAnnarosa Leri
Mar 13, 2012·Annual Review of Biophysics·Yubing SunJianping Fu
May 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Patricia E BurgerE Lynette Wilson
Aug 16, 2014·BioResearch Open Access·Sébastien SartYan Li
Sep 18, 2007·Stem Cell Reviews·François VaillantJane E Visvader
May 26, 2012·Stem Cell Reviews and Reports·Valeriya SolozobovaJan Pruszak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.