Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells

The Journal of Clinical Investigation
H MashimaI Kojima

Abstract

Rat pancreatic AR42J cells possess exocrine and neuroendocrine properties. Activin A induces morphological changes and converts them into neuron-like cells. In activin-treated cells, mRNA for pancreatic polypeptide (PP) but not that for either insulin or glucagon was detected by reverse transcription-PCR. About 25% of the cells were stained by anti-PP antibody. When AR42J cells were incubated with betacellulin, a small portion of the cells were stained positively with antiinsulin and anti-PP antibodies. The effect of betacellulin was dose dependent, being maximal at 2 nM. Approximately 4% of the cells became insulin positive at this concentration, and mRNAs for insulin and PP were detected. When AR42J cells were incubated with a combination of betacellulin and activin A, approximately 10% of the cells became insulin positive. Morphologically, the insulin-positive cells were composed of two types of cells: neuron-like and round-shaped cells. Immunoreactive PP was found in the latter type of cells. The mRNAs for insulin, PP, glucose transporter 2, and glucokinase, but not glucagon, were detected. Depolarizing concentration of potassium, tolbutamide, carbachol, and glucagon-like peptide-1 stimulated the release of immunoreactive i...Continue Reading

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G K Gittes, W J Rutter
Jan 1, 1989·Progress in Biophysics and Molecular Biology·F M Ashcroft, P Rorsman
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B WiedenmannV E Gould
Apr 22, 1988·Cell·N M Le Douarin
Feb 29, 1988·Biochemical and Biophysical Research Communications·M MurataM Muramatsu
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·L B RallW J Rutter
Dec 1, 1972·Developmental Biology·R L PictetW J Rutter
May 1, 1995·The Journal of Clinical Investigation·H OhnishiH Ishikawa
Feb 1, 1995·Endocrine Journal·M FurukawaI Kojima
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·P L HerreraJ D Vassalli
Aug 1, 1993·Endocrinology·H YasudaE Ogata
Mar 12, 1993·Science·Y ShingJ Folkman

❮ Previous
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Citations

Aug 26, 1998·International Journal of Cancer. Journal International Du Cancer·J KleeffM Korc
Feb 18, 1999·Journal of Cellular Biochemistry·H TadaH Yamada
Nov 8, 2001·Archives of Biochemistry and Biophysics·T KleinP Serup
May 10, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kristi D SilverBraxton D Mitchell
Jul 5, 2005·Journal of Hepato-biliary-pancreatic Surgery·Satoko Yamada, Itaru Kojima
Dec 17, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Mustafa KeremTonguc Utku Yilmaz
Jun 21, 2002·Biochemical and Biophysical Research Communications·Kazunori MiuraKeiichi Kawano
Sep 27, 2000·Biochimica Et Biophysica Acta·H TadaK Igarashi
May 29, 2002·Biochimica Et Biophysica Acta·Jemma LawsonAndrew J Dunbar
Aug 15, 2000·Molecular and Cellular Endocrinology·J PalgiT Otonkoski
Aug 22, 2001·Molecular and Cellular Endocrinology·Y SawadaT Takeuchi
Sep 21, 2002·Molecular and Cellular Endocrinology·Eva HofsliAstrid Laegreid
Oct 22, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·K UmezawaT-S Kam
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·A J Dunbar, C Goddard
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Seungjin ShinHee-Sook Jun
Oct 13, 2009·Diabetes, Obesity & Metabolism·C DemetercoF Levine
Dec 17, 2008·Pharmacological Reviews·Wook Kim, Josephine M Egan
Jul 17, 1998·The Journal of Clinical Investigation·T YamaokaM Itakura
Apr 4, 2007·The Journal of Clinical Investigation·Biva M DesaiDoris A Stoffers
Apr 27, 2013·The Journal of Clinical Investigation·Xiangwei XiaoGeorge K Gittes
Sep 19, 2012·Journal of Toxicologic Pathology·Hideki FujisawaKazutoshi Tamura
Jun 10, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marjorie FanjulEtienne Hollande
Oct 27, 2010·PloS One·Bela Anand-ApteJudah Folkman
Sep 11, 2007·Endocrine Journal·Akito MaeshimaYoshihisa Nojima

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