Betaine-aldehyde dehydrogenase from amaranth leaves efficiently catalyzes the NAD-dependent oxidation of dimethylsulfoniopropionaldehyde to dimethylsulfoniopropionate

Archives of Biochemistry and Biophysics
M VojtechováRosario A Muñoz-Clares

Abstract

Many flowering plants accumulate the compatible osmolyte glycine betaine in response to osmotic stress, in certain cases together with its sulfonium analog 3-dimethylsulfoniopropionate (DMSP). Compared to glycine betaine, this DMSP accumulation appears to be an evolutionary novelty. The final step in the synthesis of glycine betaine and DMSP is oxidation of the corresponding aldehyde, betaine aldehyde or 3-dimethylsulfoniopropionaldehyde (DMSPald). Leaves of amaranth (Amaranthus hypochondriacus L.) accumulate glycine betaine but do not produce detectable amounts of DMSP. These leaves contain a betaine-aldehyde dehydrogenase (BADH) that catalyzes the final step in glycine betaine synthesis. Here we report that this enzyme efficiently catalyzes the oxidation of DMSPald. On the basis of Vmax/Km values, DMSPald is a better substrate for amaranth BADH than betaine aldehyde itself. The kinetic mechanism followed by amaranth BADH at low concentrations of DMSPald is similar to that with betaine aldehyde; as determined from initial velocity, product, dead-end, and substrate inhibition studies, it is a steady-state bi bi with ordered addition of substrates and random release of products. Like betaine aldehyde, DMSPald is inhibitory at hi...Continue Reading

Citations

Aug 31, 2004·Plant Physiology·Sigrun ReumannLavanya Babujee
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·N. TerryA. S. Tarun
Nov 26, 2008·BMC Genomics·Carlos MolinaPeter Winter
Dec 12, 2012·Chemico-biological Interactions·Héctor Riveros-RosasRosario A Muñoz-Clares
Dec 1, 2012·Free Radical Biology & Medicine·Surendra SinghVasilis Vasiliou
Nov 1, 2005·Journal of Plant Physiology·Hideki Oishi, Masumi Ebina
Oct 24, 2001·Plant & Cell Physiology·T NakamuraT Takabe
Feb 11, 1999·Journal of Bacteriology·R Velasco-GarcíaR A Muñoz-Clares
Sep 27, 2000·Biochimica Et Biophysica Acta·M SebelaP Pec
Sep 22, 2009·Archives of Biochemistry and Biophysics·Rosario A Muñoz-ClaresCarmina Montiel
Jan 9, 2003·Chemical Reviews·Thomas G Chasteen, Ronald Bentley

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