Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

BMC Veterinary Research
Jingtao WuXiaoping Yang

Abstract

The intestinal epithelial barrier, which works as the first line of defense between the luminal environment and the host, once destroyed, it will cause serious inflammation or other intestinal diseases. Tight junctions (TJs) play a vital role to maintain the integrity of the epithelial barrier. Lipopolysaccharide (LPS), one of the most important inflammatory factors will downregulate specific TJ proteins including Occludin and Claudin-1 and impair integrity of the epithelial barrier. Betaine has excellent anti-inflammatory activity but whether betaine has any effect on TJ proteins, particularly on LPS-induced dysfunction of epithelial barriers remains unknown. The purpose of this study is to explore the pharmacological effect of betaine on improving intestinal barrier function represented by TJ proteins. Intestinal porcine epithelial cells (IPEC-J2) were used as an in vitro model. The results demonstrated that betaine enhanced the expression of TJ proteins while LPS (1 μg/mL) downregulates the expression of these proteins. Furthermore, betaine attenuates LPS-induced decreases of TJ proteins both shown by Western blot (WB) and Reverse transcription-polymerase chain reaction (RT-PCR). The immunofluorescent images consistently rev...Continue Reading

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Citations

Sep 3, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lei GeKehe Huang
Dec 10, 2020·Animals : an Open Access Journal From MDPI·Dennis E Jewell, Matthew I Jackson
Apr 4, 2021·International Journal of Molecular Sciences·Tracy ParadisFabienne Bon
May 1, 2021·Toxins·Haoming WuJiaqi Wang
Jun 3, 2021·Biology·Madan Kumar ArumugamKusum K Kharbanda
Nov 9, 2021·The British Journal of Nutrition·Damoon Ashtary-LarkyAlexei Wong

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Methods Mentioned

BETA
environmental stress
electrophoresis
light contrast microscopy
PCR

Software Mentioned

GraphPad Prism
Graphpad Prism6
Image J

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