Betaine protects against heat exposure-induced oxidative stress and apoptosis in bovine mammary epithelial cells via regulation of ROS production.

Cell Stress & Chaperones
Chengmin LiGenlin Wang

Abstract

Heat stress is one of the wide varieties of factors which cause oxidative stress in vivo; elevated temperature can lead to oxidative stress of dairy cows that affects milk production. The aim of this study was to determine the capacity of the betaine to act as an antioxidant against oxidative stress induced by heat exposure and apoptosis in mammary epithelial cells (mammary alveolar cells, MAC-T). The MAC-T were divided into four treatment groups: control (37 °C), heat stress (HS, 42 °C), betaine (37 °C), and HS + betaine. MAC-T under heat stress (HS) showed increased ROS accumulation, malondialdehyde (MDA) content, superoxide dismutase (SOD) concentration, and catalase (CAT) activity. During heat stress, betaine decreased the mRNA expression level of HSP70 and HSP27 in MAC-T. Bax/Bcl-2 ratio and caspase-3, the markers of apoptosis, were also elevated in MAC-T under heat stress. The markers of oxidative stress Nrf-2/HO-1 genes were also elevated in MAC-T under heat stress. Pretreatment of betaine reversed the heat-induced depletion in total antioxidant status, ROS accumulation, and SOD and CAT contents in MAC-T. Bax/Bcl-2 ratio and Nrf-2/HO-1 expression of heat-exposed MAC-T were also reduced with betaine supplementation. In co...Continue Reading

References

Mar 2, 2002·Stroke; a Journal of Cerebral Circulation·Chih-Ya Yang, Mao-Tsun Lin
Oct 27, 2007·Journal of the American College of Nutrition·Florian Lang
Jun 1, 2005·Nutrition Research Reviews·M EklundR Mosenthin
Jul 31, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Hector Macias, Lindsay Hinck
Oct 16, 2014·Animal : an International Journal of Animal Bioscience·D L Hill, E Wall
Oct 31, 2014·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Imen Belhadj SlimenManef Abdrabbah
Dec 3, 2014·Chemico-biological Interactions·Volodymyr I Lushchak
Apr 22, 2016·Cellular and Molecular Life Sciences : CMLS·Agnieszka LobodaJozef Dulak
May 5, 2016·Journal of Agricultural and Food Chemistry·Mengmeng ZhangTian Min
Oct 11, 2016·Journal of Dairy Science·L W HallR J Collier
Jul 25, 2017·Tropical Animal Health and Production·Muhammad SaeedSun Chao
Apr 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Reza HeidariAkram Jamshidzadeh
Jun 29, 2018·Journal of the Science of Food and Agriculture·Chao WenYanmin Zhou

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Citations

Jul 17, 2020·Frontiers in Cell and Developmental Biology·Zhihui WuShihai Zhang
Nov 8, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Hanako BaiMasashi Takahashi
Nov 17, 2020·In Vitro Cellular & Developmental Biology. Animal·Nungnuch SaipinRuttachuk Rungsiwiwut
Sep 9, 2020·Asian-Australasian Journal of Animal Sciences·Md Aminul IslamShinichi Yonekura

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Methods Mentioned

BETA
environmental stresses
FACS
Assay
protein assay
MDA
flow cytometry

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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