Betaine suppresses cell proliferation by increasing oxidative stress-mediated apoptosis and inflammation in DU-145 human prostate cancer cell line

Cell Stress & Chaperones
Fatih KarGungor Kanbak

Abstract

Prostate cancer is the main cause of cancer-related mortality in men around the world and an important health problem. DU-145 human prostate cancer cells provide an opportunity to investigate prostate cancer. Betaine has a number of anticancer effects, such as inactivation of carcinogens, inhibition of cancer cell proliferation, angiogenesis, and metastasis. However, there is no study investigating the effects of betaine on DU-145 cells. The aim of this study was to evaluate the effects of different concentrations of betaine on the oxidative stress, apoptosis, and inflammation on DU-145 cells. Firstly, we proved the cytotoxic activity of betaine (0 to 150 mg/ml) on DU-145 cells by using 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and defined the optimal concentration of betaine. Then, by employing the doses found in MTT, the levels of antioxidant (GSH, SOD, CAT, and TAS) and oxidant (MDA and TOS) molecules, pro-inflammatory cytokines (TNF-a and IL-6), apoptotic proteins (CYCS and CASP3), and DNA fragmentation were measured. Morphological changes and apoptosis were evaluated using H&E technique, Bax and Bcl-2 immunohistochemistry. Results suggested that betaine caused oxidative stress, inflammation, in...Continue Reading

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Citations

Feb 20, 2021·Journal of Biochemical and Molecular Toxicology·Fatih KarVarol Sahinturk
Feb 6, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoting XiYan Li
May 19, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Mohammed Al Za'abiBadreldin H Ali

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Methods Mentioned

BETA
Assay
ELISA
MDA

Software Mentioned

SPSS
- Pad Prism
Graph

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