Betaine tripled the volumetric productivity of D(-)-lactate by Escherichia coli strain SZ132 in mineral salts medium

Biotechnology Letters
S ZhouL O Ingram

Abstract

Osmotic stress restricts glycolytic flux, growth (rate and yield), D-lactate productivity, and D-lactate tolerance in Escherichia coli B strain SZ132 during batch fermentation in mineral salts medium with 10% (w/v) sugar. Addition of 1 mM: betaine, a non-metabolized protective osmolyte, doubled cell yield, increased specific productivity of D-lactate and glycolytic flux by 50%, and tripled volumetric productivity (from 8.6 to 25.7 mmol l(-1) h(-1); 0.8 to 2.3 g l(-1) h(-1)). Glycolytic flux and specific productivity in mineral salts medium with betaine exceeded that in Luria broth, substantially eliminating the need for complex nutrients during D-lactate production. In mineral salts medium supplemented with betaine, SZ132 produced approximately 1 mol D-lactate (90 g) per 100 g sugar (glucose or sucrose).

References

Aug 24, 1999·Journal of Industrial Microbiology & Biotechnology·S Zhou, L Ingram
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·T B CauseyL O Ingram
Jun 16, 2004·Biotechnology Letters·V B ShuklaL O Ingram
Oct 7, 2004·Macromolecular Bioscience·Sang Y LeeSi J Park
Jan 27, 2005·Analytical and Bioanalytical Chemistry·Shenguo WangJianzhong Bei
Jul 8, 2005·Applied and Environmental Microbiology·J E PurvisL O Ingram

❮ Previous
Next ❯

Citations

Sep 18, 2007·Applied Microbiology and Biotechnology·Xueli ZhangL O Ingram
May 28, 2009·Journal of Industrial Microbiology & Biotechnology·José UtrillaAlfredo Martinez
Dec 17, 2009·Journal of Industrial Microbiology & Biotechnology·Yihui ZhuElliot Altman
Dec 13, 2006·Biotechnology Letters·Alfredo MartinezL O Ingram
Sep 6, 2008·Biotechnology Letters·L P YomanoL O Ingram
Sep 8, 2009·Applied and Environmental Microbiology·Cong T Trinh, Friedrich Srienc
Sep 23, 2008·Applied and Environmental Microbiology·Yihui ZhuElliot Altman
Mar 20, 2008·Journal of Molecular Microbiology and Biotechnology·K T Shanmugam, L O Ingram
Apr 23, 2009·Microbial Cell Factories·Susana Romero-GarciaAlfredo Martinez
Jul 7, 2011·Journal of Biotechnology·Mohamed Ali Abdel-RahmanKenji Sonomoto
Mar 24, 2016·Applied Microbiology and Biotechnology·Huibin ZouJunhong Liu
Oct 16, 2015·Preparative Biochemistry & Biotechnology·Gong ChengWeiguo Zhang
Oct 30, 2012·Trends in Biotechnology·Rebecca M Lennen, Brian F Pfleger
Feb 5, 2013·Metabolic Engineering·Lauren B A WoodruffRyan T Gill
Oct 8, 2017·Biotechnology and Bioengineering·Deepika AwasthiKeelnatham T Shanmugam
Aug 1, 2018·Bioprocess and Biosystems Engineering·Yuewen SuJian Wang
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Liang WangK T Shanmugam
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Fan SuoPeter R Jensen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.