Betanodavirus non-structural protein B2: A novel necrotic death factor that induces mitochondria-mediated cell death in fish cells

Virology
Yu-Chin SuJiann-Ruey Hong

Abstract

The Betanodavirus non-structural protein B2 plays a role in silencing RNA interference (RNAi), which mediated regulation of animal and plant innate immune responses, but little is known regarding the role of B2 in cell death. The present study examined the effects of B2 on mitochondria-mediated necrotic cell death in grouper liver (GL-av) cells. B2 was expressed at 12 h post-infection (pi), with increased expression between 24 and 72 h pi by Western blot. B2 was transiently expressed to investigate possible novel protein functions. Transient expression of B2 in GL-av cells resulted in apoptotic cell features and positive TUNEL assays (28%) at 24 h post-transfection (pt). During mechanistic studies of cell death, B2 upregulated expression of the proapoptotic gene Bax (2.8 fold at 48 h pt) and induced loss of mitochondria membrane potential (MMP) but not mitochondrial cytochrome c release. Furthermore, over expression of Bcl-2 family member zfBcl-xL effectively prevented B2-induced, mitochondria-mediated necrotic cell death. Finally, using RNA interference to reduce B2 expression, both B2 and Bax expression were downregulated and RGNNV-infected cells were rescued from secondary necrosis. Taken together, our results suggest that B...Continue Reading

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Citations

Jun 6, 2014·International Journal of Cell Biology·Mohammad Latif ReshiJiann-Ruey Hong
Feb 15, 2011·Veterinary Research·Stéphane Biacchesi
Dec 21, 2010·Apoptosis : an International Journal on Programmed Cell Death·Yu-Chin SuJiann-Ruey Hong
Jan 26, 2010·Developmental and Comparative Immunology·María P SepulcreVictoriano Mulero
Oct 24, 2015·Virus Research·Latif ReshiJiann-Ruey Hong
Jun 20, 2014·Fish & Shellfish Immunology·Mohammad Latif ReshiJiann-Ruey Hong
Dec 17, 2014·Molecular Phylogenetics and Evolution·Mei He, Chun-Bo Teng
Oct 25, 2011·Developmental and Comparative Immunology·Aina-Cathrine OvergårdSonal Patel
Sep 2, 2016·Developmental and Comparative Immunology·Yuan-Kai ChengShau-Chi Chi
Apr 28, 2017·Journal of Fish Diseases·C-F LowM Najiah
Feb 14, 2020·Pathogens·Isabel Bandín, Sandra Souto

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