Betula platyphylla BpHOX2 transcription factor binds to different cis-acting elements and confers osmotic tolerance.

Journal of Integrative Plant Biology
Zilong TanYucheng Wang

Abstract

The homeodomain-leucine zipper (HD-Zip) proteins play crucial roles in plant developmental and environmental responses. However, how they mediate gene expression to facilitate abiotic stress tolerance remains unknown. In the present study, we characterized BpHOX2 (encoding a HD-Zip I family protein) from birch (Betula platyphylla). BpHOX2 is predominately expressed in mature stems and leaves, but expressed at a low level in apical buds and roots, suggesting that it has tissue-specific characteristics. BpHOX2 expression was highly induced by osmotic and salt, but only slightly induced by abscisic acid. Overexpression of BpHOX2 markedly improved osmotic tolerance, while knockdown of BpHOX2 increased sensitivity to osmotic stress. BpHOX2 could induce the expression of pyrroline-5-carboxylate synthase, peroxidase, and superoxide dismutase genes to improve proline levels and the reactive oxygen species scavenging capability. Chromatin immunoprecipitation sequencing combined with RNA sequencing showed that BpHOX2 could bind to at least four cis-acting elements, including dehydration-responsive element "RCCGAC", Myb-p binding box "CCWACC," and two novel cis-acting elements with the sequences of "AAGAAG" and "TACGTG" (termed HBS1 and H...Continue Reading

References

Sep 13, 2001·Plant Physiology·R DesikanS J Neill
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Dec 20, 2005·Plant Cell Reports·Violeta Andjelkovic, Richard Thompson
Aug 19, 2007·Trends in Plant Science·Federico D ArielRaquel L Chan
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Aug 10, 2010·BMC Bioinformatics·Mali Salmon-DivonPaul Bertone
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Jun 25, 2013·Plant, Cell & Environment·Polavarapu B Kavi Kishor, Nese Sreenivasulu
Feb 18, 2014·Journal of Integrative Plant Biology·Ronny BrandtStephan Wenkel
Jul 31, 2014·Plant Molecular Biology·Guoliang HanBaoshan Wang
Apr 26, 2015·Journal of Experimental Botany·L TurchiI Ruberti
Aug 16, 2016·The New Phytologist·Farshad Roodbarkelari, Edwin P Groot
Sep 15, 2017·Plant Biotechnology Journal·Yingying ZhuLaigeng Li
Dec 19, 2018·International Journal of Molecular Sciences·Giovanna SessaIda Ruberti
Jun 27, 2019·International Journal of Molecular Sciences·Xuejing WenYucheng Wang

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