Betulin alleviated ethanol-induced alcoholic liver injury via SIRT1/AMPK signaling pathway

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Ting BaiJi-Xing Nan

Abstract

The present study was conducted to investigate the protective effect of betulin, a triterpene from the bark of Betula platyphylla Suk, against ethanol-induced alcoholic liver injury and its possible underlying mechanisms. In vitro, human hepatic stellate cell line, LX-2 cells were treated with betulin (6.25, 12.5 and 25 μM) prior to ethanol (50mM) for 24h. Cell viability was analyzed by methyl thiazolyl tetrazolium assay, protein expressions were assessed by Western blot. In vivo, we induced alcoholic liver injury in male C57BL/6 mice, placing them on Lieber-DeCarli ethanol-containing diets for 10 days and then administering a single dose of ethanol (5 g/kg body weight) via gavage. Betulin (20 and 50mg/kg) were given by gavage every day. In vitro results showed that betulin effectively decreased LX-2 cell viability, attenuated collagen-I, α-smooth muscle actin (α-SMA) levels, activated liver kinase B-1 (LKB1) and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation. Betulin suppressed the expression of sterol regulatory element-binding protein-1 (SREBP-1), and genetic deletion of AMPK blocked the effect of betulin on SREBP-1 in ethanol treated LX-2 cells. In vivo, betulin attenuated the increases in serum ami...Continue Reading

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Citations

Jan 10, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ho Jong RaSun-Chul Hwang
Dec 19, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Qiong-Hui HuangYou-Liang Xie
May 16, 2018·American Journal of Physiology. Endocrinology and Metabolism·Greta TrogenJonathan M Peterson
May 30, 2019·Alcoholism, Clinical and Experimental Research·Chi LiuXiao-Kang Li
Mar 4, 2020·Expert Review of Gastroenterology & Hepatology·Liping ChenDesong Kong
Jul 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaowei HuoYanyan Zhao
May 18, 2020·Natural Products and Bioprospecting·Peng-Hui YuanGuan-Hua Du
Aug 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing-Yi QiaoMing-San Miao
Jun 20, 2017·Oxidative Medicine and Cellular Longevity·Sijie Tan, Esther Wong
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gonçalo P RosaMaria Carmo Barreto
Jul 3, 2021·Journal of Ethnopharmacology·Myong Hak RiXuejun Jin
Jul 26, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lina KongQiang Meng
Apr 16, 2017·Gene Expression·Bin GaoSuthat Liangpunsakul

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