Beware biofilm! Dry biofilms containing bacterial pathogens on multiple healthcare surfaces; a multi-centre study

The Journal of Hospital Infection
K LedwochJ-Y Maillard

Abstract

Wet biofilms associated with medical devices have been widely studied and their link with healthcare-associated infections (HCAIs) is well recognized. Little attention has been paid to the presence of dry biofilms on environmental surfaces in healthcare settings. To investigate the occurrence, prevalence, and diversity of dry biofilms on hospital surfaces. Sixty-one terminally cleaned items were received from three different UK hospitals. The presence of dry biofilm was investigated using culture-based methods and scanning electron microscopy (SEM). Bacterial diversity within biofilms was investigated using ribosomal RNA intergenic spacer analysis (RISA)-polymerase chain reaction and next-generation sequencing. Multi-species dry biofilms were recovered from 95% of 61 samples. Abundance and complexity of dry biofilms were confirmed by SEM. All biofilms harboured Gram-positive bacteria including pathogens associated with HCAI; 58% of samples grew meticillin-resistant Staphylococcus aureus. Dry biofilms had similar physical composition regardless of the type of items sampled or the ward from which the samples originated. There were differences observed in the dominance of particular species: dry biofilms from two hospitals contain...Continue Reading

Citations

Feb 26, 2019·Letters in Applied Microbiology·K LedwochJ-Y Maillard
Sep 19, 2019·Foodborne Pathogens and Disease·Alejandra Andrea LatorreMarcos Alberto Muñoz
Mar 1, 2020·Journal of Fungi·Ryan KeanGordon Ramage
Dec 24, 2018·Materials·Katarzyna Ledwoch, Jean-Yves Maillard
Sep 25, 2020·Annals of Work Exposures and Health·Anne Mette MadsenKatrine Uhrbrand
Oct 22, 2020·Antimicrobial Resistance and Infection Control·Philip BowlerRandall Wolcott
Oct 5, 2020·Infection, Disease & Health·Brett G MitchellCassie Curryer
Feb 20, 2021·Expert Review of Vaccines·Abraham Loera-MuroCarlos Angulo
Mar 3, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Katarzyna LedwochJean-Yves Maillard
Mar 2, 2021·The Journal of Hospital Infection·K LedwochJ-Y Maillard
May 12, 2021·Antimicrobial Resistance and Infection Control·Wing-Kee YiekAlma Tostmann
Jul 15, 2021·Revista brasileira de enfermagem·Elaine Aparecida da Silva NascimentoJussimara Monteiro
Oct 27, 2021·Critical Reviews in Microbiology·Wolfgang BäumlerWulf Schneider-Brachert
Jun 23, 2020·American Journal of Infection Control·John M Boyce

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