Beyond genomics: critical evaluation of cell line utility for ovarian cancer research

Gynecologic Oncology
Kevin M EliasRonny Drapkin

Abstract

Comparisons of The Cancer Genome Atlas (TCGA) with high grade serous ovarian cancer (HGSOC) cell lines used in research reveal that many common experimental models lack defining genomic characteristics seen in patient tumors. As cell lines exist with higher genomic fidelity to TCGA, this study aimed to evaluate the utility of these cell lines as tools for preclinical investigation. We compared two HGSOC cell lines with supposed high genomic fidelity to TCGA, KURAMOCHI and OVSAHO, with the most commonly cited ovarian cancer cell line, SKOV3, which has poor genomic fidelity to TCGA. The lines were analyzed for genomic alterations, in vitro performance, and growth in murine xenografts. Using targeted next generation sequencing analyses, we determined that each line had a distinct mutation profile, including alterations in TP53, and copy number variation of specific genes. KURAMOCHI and OVSAHO better recapitulated serous carcinoma morphology than SKOV3. All lines expressed PAX8 and stathmin, but KURAMOCHI and OVSAHO did not express CK7. KURAMOCHI was significantly more platinum sensitive than OVSAHO and SKOV3. Unlike SKOV3, KURAMOCHI and OVSAHO engrafted poorly in subcutaneous xenografts. KURAMOCHI and OVSAHO grew best after intrap...Continue Reading

References

Jan 1, 1989·Cancer Chemotherapy and Pharmacology·M M Tomayko, C P Reynolds
Apr 1, 1986·Journal of Surgical Oncology·D M EuhusF E Johnson
Sep 1, 2012·Nature Protocols·Alison M Karst, Ronny Drapkin
Jul 11, 2013·Nature Communications·Silvia DomckeNikolaus Schultz
Sep 12, 2013·PloS One·Michael S AnglesioDavid G Huntsman
Oct 12, 2013·PloS One·Michael S AnglesioDavid G Huntsman
Nov 8, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Young-Ae ParkDuk-Soo Bae
Jan 9, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·S John WerohaPaul Haluska
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 7, 2015·Cold Spring Harbor Protocols·Jen-Chieh Tseng, Andrew L Kung
Apr 17, 2015·Science Translational Medicine·Siân JonesVictor E Velculescu
Jun 9, 2015·Gynecologic Oncology·Anirban K MitraJoanna E Burdette

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Citations

May 29, 2016·Gynecologic Oncology·Lidia HernandezChristina M Annunziata
Feb 18, 2017·International Journal of Molecular Sciences·Razmik MirzayansDavid Murray
Mar 4, 2017·Journal of the American Chemical Society·Zhaoqianqi FengBing Xu
Jul 19, 2018·International Journal of Molecular Sciences·Patrycja TudrejKatarzyna M Lisowska
Jan 30, 2018·Bioengineering & Translational Medicine·Erik C DreadenPaula T Hammond
Feb 28, 2016·BMC Cancer·Hope MurphyHana M Dobrovolny
Mar 23, 2017·Molecular Cancer·Michela Lupia, Ugo Cavallaro
Oct 19, 2017·International Journal of Oncology·Petar OzretićMaja Sabol
Jun 9, 2018·Scientific Reports·N HojoJ J Unternaehrer
Apr 26, 2020·Cell Death & Disease·Boxiao DingSteven R Grossman
Mar 1, 2019·International Journal of Molecular Sciences·Michael-Antony LisioCarlos Telleria
Oct 11, 2020·Journal of Hematology & Oncology·Meinusha GovindarajanThomas Kislinger
Sep 25, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·George Au-YeungDavid D L Bowtell
Aug 31, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joyce F LiuRonny Drapkin

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