Beyond knockouts: the International Knockout Mouse Consortium delivers modular and evolving tools for investigating mammalian genes

Mammalian Genome : Official Journal of the International Mammalian Genome Society
Barry RosenWolfgang Wurst

Abstract

The International Knockout Mouse Consortium (IKMC; http://www.mousephenotype.org ) has generated mutations in almost every protein-coding mouse gene and is completing the companion Cre driver resource to expand tissue-specific conditional mutagenesis. Accordingly, the IKMC has carried out high-throughput gene trapping and targeting producing conditional mutations in murine embryonic stem cells in more than 18,500 genes, from which at least 4900 mutant mouse lines have been established to date. This resource is currently being upgraded with more powerful tools, such as visualization and manipulation cassettes that can be easily introduced into IKMC alleles for multifaceted functional studies. In addition, we discuss how existing IKMC products can be used in combination with CRISPR technology to accelerate genome engineering projects. All information and materials from this extraordinary biological resource together with coordinated phenotyping efforts can be retrieved at www.mousephenotype.org . The comprehensive IKMC knockout resource in combination with an extensive set of modular gene cassettes will continue to enhance functional gene annotation in the future and solidify its impact on biomedical research.

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Citations

Jun 9, 2016·Pediatric Neurology·Anne M ComiUNKNOWN 2015 Sturge-Weber Syndrome Research Workshop
May 22, 2016·Journal of Neurochemistry·Daniela Maria Vogt WeisenhornWolfgang Wurst
Oct 25, 2014·Experimental Animals·Kazuyuki MekadaAtsushi Yoshiki
Sep 2, 2016·Scientific Reports·Joel A SchickWolfgang Wurst
Nov 2, 2017·Nucleic Acids Research·Cynthia L SmithUNKNOWN Mouse Genome Database Group
Apr 11, 2018·Human Mutation·Samya Chakravorty, Madhuri Hegde
Sep 2, 2016·Current Protocols in Mouse Biology·Anna MikhalevaBinnaz Yalcin
Jul 6, 2017·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Marie-Christine BirlingGuillaume Pavlovic
Jan 11, 2019·Disease Models & Mechanisms·Channabasavaiah B Gurumurthy, Kevin C Kent Lloyd
May 28, 2019·Frontiers in Endocrinology·Robert D Maynard, Cheryl L Ackert-Bicknell
Mar 18, 2021·Disease Models & Mechanisms·Jens HansenWolfgang Wurst

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