Apr 29, 2020

Abundance and diversity of phages, microbial taxa and antibiotic resistance genes in the sediments of the river Ganges through metagenomic approach

BioRxiv : the Preprint Server for Biology
Manoj KumarManoj Kumar

Abstract

In the present study, we have analyzed the metagenomic DNA from the pooled sediment sample of the river Ganges to explore the abundance and diversity of phages, microbial community and antibiotic resistance genes. Utilizing data from Illumina platform, 4174 (~0.0013%) reads were classified for the 285 different DNA viruses largely dominated by the group of 260 distinctive phages (3602 reads, ~86.3%). Among all, Microcystis (782 hits), Haemophilus (403), Synechococcus (386), Pseudomonas (279), Enterococcus (232), Bacillus (196), Rhodococcus (166), Caulobacter (163), Salmonella (146), Enterobacteria (143), Mycobacterium (128), Propionibacterium (71), Erwinia (70), Ralstonia (56) phages shows the highest abundance and account for approximately 90% of the total identified phages. Additionally, we have also identified corresponding host pertaining to these phages. Mainly, Proteobacteria (~69.3%) dominates the microbial population structure. Primarily orders such as Caulobacterales (~28%), Burkholderiales (~13.9%), Actinomycetales (~13.7%), Pseudomonadales (~7.5%) signify the core section. Further, 21869 (~0.00695%) reads were classified in 20 ARG types (classes) and 240 ARGs (subtypes) among which 4 ARG types namely multidrug resist...Continue Reading

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Mentioned in this Paper

Biochemical Pathway
Size
Reconstructive Surgical Procedures
Anatomical Space Structure
Potassium Ion
Nucleotides
Local
Genotype Determination
Orientation (spatial)
Biological Evolution

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