PMID: 8944746Nov 11, 1996Paper

Bezafibrate and clofibric acid are novel inhibitors of phosphatidylcholine synthesis via the methylation of phosphatidylethanolamine

Biochimica Et Biophysica Acta
T Nishimaki-MogamiA Takahashi

Abstract

The effects of bezafibrate and clofibric acid, fibrate hypolipidemic agents, on phosphatidylcholine (PC) synthesis via the phosphatidylethanolamine (PE) methylation pathway were studied. In cultured rat hepatocytes, bezafibrate and clofibric acid added to the medium rapidly and markedly reduced the conversion of ethanolamine-labeled PE to PC (IC50 30 and 150 microM, respectively). Furthermore, the methylation of PE derived from serine was also blocked by bezafibrate, as was the secretion of PC derived from either serine or ethanolamine. The microsomal activity of PE N-methyltransferase was inhibited by these agents. Perfluorooctanoic acid but not DCQVA, though both are potent peroxisome proliferators comparable to fibrates, produced this inhibition. The inhibitory effects produced by these agents were diminished by dithiothreitol (DTT) added to the assay or alkaline pH assay condition. Inhibition by oleic acid was also attenuated under these conditions, suggesting a common mechanism of inhibition. However, unlike fatty acids, fibrates did not have rapid stimulatory effects on the CDP-choline pathway in hepatocytes. These results suggest that fibrates may mimic fatty acids in regulating PC synthesis from the PE methylation pathw...Continue Reading

References

Jun 15, 1979·Biochemical Pharmacology·P K Chiang, G L Cantoni
Feb 4, 1992·Biochemical Pharmacology·S Green
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M GöttlicherJ A Gustafsson
Jan 1, 1989·Annual Review of Pharmacology and Toxicology·E A LockC R Elcombe
Oct 1, 1986·The Journal of Pharmacy and Pharmacology·M J Van der MeerF O Müller
Jan 1, 1988·Progress in Lipid Research·D E Vance, N D Ridgway
Nov 1, 1986·The Biochemical Journal·M BronfmanM N Morales
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S ParthasarathyW J Baumann
Jun 25, 1984·Biochimica Et Biophysica Acta·S L Pelech, D E Vance
Oct 13, 1995·The Journal of Biological Chemistry·K YuM A Lazar
May 11, 2004·The Journal of Organic Chemistry·Emília ValenteJim Iley

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Citations

May 9, 1998·Toxicology Letters·T MurakamiK Hirano
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·C J WalkeyD E Vance
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gabriella PessiChoukri Ben Mamoun
Apr 19, 2006·In Vitro Cellular & Developmental Biology. Animal·Hisae Kume, Hajime Sasaki
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Hiroko KawanoMasaharu Miyake
Apr 29, 1998·Current Opinion in Lipidology·D E Vance, C J Walkey
Nov 15, 2002·The Journal of Biological Chemistry·David J ShieldsDennis E Vance
Jul 5, 2003·The Journal of Biological Chemistry·David J ShieldsDennis E Vance

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