bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation

Development
Evanthia ZacharioudakiChristos Delidakis

Abstract

Drosophila larval neurogenesis is an excellent system for studying the balance between self-renewal and differentiation of a somatic stem cell (neuroblast). Neuroblasts (NBs) give rise to differentiated neurons and glia via intermediate precursors called GMCs or INPs. We show that E(spl)mγ, E(spl)mβ, E(spl)m8 and Deadpan (Dpn), members of the basic helix-loop-helix-Orange protein family, are expressed in NBs but not in differentiated cells. Double mutation for the E(spl) complex and dpn severely affects the ability of NBs to self-renew, causing premature termination of proliferation. Single mutations produce only minor defects, which points to functional redundancy between E(spl) proteins and Dpn. Expression of E(spl)mγ and m8, but not of dpn, depends on Notch signalling from the GMC/INP daughter to the NB. When Notch is abnormally activated in NB progeny cells, overproliferation defects are seen. We show that this depends on the abnormal induction of E(spl) genes. In fact E(spl) overexpression can partly mimic Notch-induced overproliferation. Therefore, E(spl) and Dpn act together to maintain the NB in a self-renewing state, a process in which they are assisted by Notch, which sustains expression of the E(spl) subset.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C Delidakis, S Artavanis-Tsakonas
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·P AlifragisC Delidakis
Jan 27, 1998·Developmental Biology·L SeugnetM Haenlin
Sep 12, 1998·Developmental Biology·S Fuerstenberg, E Giniger
Jun 22, 1999·Molecular and Cellular Biology·B H JenningsS J Bray
Dec 6, 2002·Development·Nikolaos GiagtzoglouChristos Delidakis
Jan 28, 2003·Journal of Cellular Physiology·Tatsuya IsoYasuo Hamamori
Oct 28, 2004·The Journal of Biological Chemistry·Nikolaos GiagtzoglouChristos Delidakis
May 27, 2005·Nature Neuroscience·Keejung Yoon, Nicholas Gaiano
Nov 9, 2005·Mechanisms of Development·Mara S Almeida, Sarah J Bray
Dec 22, 2006·Genes & Development·Hongyan WangWilliam Chia
Mar 3, 2007·Development·Ryoichiro KageyamaTaeko Kobayashi
Jun 26, 2007·Nucleic Acids Research·Andreas Fischer, Manfred Gessler
Feb 26, 2008·Cell·Juergen A Knoblich
Mar 11, 2008·Seminars in Cell & Developmental Biology·Pao-Shu WuAndrea H Brand
Mar 18, 2008·Developmental Cell·Sarah K BowmanJuergen A Knoblich
Apr 24, 2008·Development, Growth & Differentiation·Ryoichiro KageyamaTaeko Kobayashi
Jan 30, 2009·Science Signaling·Alena KrejcíSarah J Bray
Dec 17, 2009·Neural Development·Natalya IzerginaHeinrich Reichert
Mar 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Itaru ImayoshiRyoichiro Kageyama
Sep 8, 2010·Current Topics in Developmental Biology·Sarah Bray, Fred Bernard

❮ Previous
Next ❯

Citations

Sep 6, 2012·Molecular and Cellular Biology·Ioanna ZarifiChristos Delidakis
Oct 3, 2013·PloS One·A Burcu BabaoğlanSarah J Bray
Mar 19, 2014·Seminars in Cell & Developmental Biology·Derek H Janssens, Cheng-Yu Lee
Jun 12, 2014·Applied Biochemistry and Biotechnology·Min LiuZuoming Nie
Apr 29, 2014·Journal of Neurogenetics·Yanrui Jiang, Heinrich Reichert
Jan 29, 2016·Cellular and Molecular Life Sciences : CMLS·Francesca Froldi, Louise Y Cheng
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tsz-Leung ToXiaokun Shu
Jun 11, 2015·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ömür Y Tastan, Ji-Long Liu
Oct 1, 2015·Nature Reviews. Neuroscience·Catarina C F HomemJürgen A Knoblich
Jan 31, 2015·Cell Death and Differentiation·R AryaK White
Feb 20, 2015·Nucleic Acids Research·Marianthi KiparakiChristos Delidakis
Aug 27, 2014·PloS One·Alexandre DjianeSarah J Bray
Jan 30, 2019·ELife·Evanthia ZacharioudakiSarah Bray
Nov 24, 2018·Neural Development·Esteban G ContrerasAndrea H Brand
Oct 24, 2018·Journal of Developmental Biology·Katherine Harding, Kristin White
Mar 30, 2017·Developmental Cell·Kun LiuYan Song
Nov 8, 2012·Development·Catarina C F Homem, Juergen A Knoblich
Jan 17, 2015·Genes & Development·Francesca FroldiLouise Y Cheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.