bHLH093/NFL and bHLH061 are required for apical meristem function in Arabidopsis thaliana

Plant Signaling & Behavior
B C PoirierB M Lange

Abstract

The basic Helix-Loop-Helix (bHLH) transcription factors SCREAM/ICE1 and SCREAM2 have well-characterized roles in the terminal differentiation of stomatal guard cells in Arabidopsis thaliana. Here we report on the characterization of the functional roles of the remaining members of sub-group IIIB, bHLH093 and bHLH061. The bhlh093/bhlh061 double mutant failed to produce a primary inflorescence shoot and displayed greater phenotypic severity than bhlh093 and bhlh061 single mutants. An ultrastructural investigation revealed structural defects that develop in tissues surrounding the meristem prior to inflorescence emergence. The transition to flowering was restored in bhlh093/bhlh061 with the application of gibberellin to the apex. We also demonstrate that gibberellin application alleviates structural defects that develop in tissues surrounding the meristem and restore meristem activity. Furthermore, the bhlh093/bhlh061 double mutant was affected by delayed flowering, and the severity of the phenotype correlated with photoperiod and light intensity. Our results indicate that bHLH093 and bHLH061 function in the gibberellin-mediated establishment of functional apical meristems during the transition from vegetative to reproductive growth.

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
May 31, 2003·International Journal of Environmental Health Research·V Curtis
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Aug 13, 2003·Journal of Molecular Evolution·Michael J Buck, William R Atchley
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Wilhelm Rademacher
Jun 10, 2004·Genome Biology·Susan Jones
Nov 4, 2004·Biochemical and Biophysical Research Communications·Kin Tak Chu, Tzi Bun Ng
Jun 18, 2005·The Plant Journal : for Cell and Molecular Biology·Kiana ToufighiNicholas J Provart
Nov 1, 1992·Plant Physiology·W M ProebstingL N Proebsting
Nov 1, 1987·Plant Physiology·S J Crafts-Brandner, D B Egli
Aug 1, 2006·Current Opinion in Plant Biology·Eilon ShaniNaomi Ori
Nov 8, 2006·The Plant Cell·Kyoko Ohashi-Ito, Dominique C Bergmann
Nov 9, 2007·The Plant Journal : for Cell and Molecular Biology·Britta RohdeUlrich Matern
Jan 5, 2008·Annual Review of Plant Biology·Shinjiro Yamaguchi
Oct 22, 2008·Journal of Experimental Botany·Tamotsu Hisamatsu, Rod W King
Mar 7, 2009·Journal of Experimental Botany·Effie Mutasa-Göttgens, Peter Hedden
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sean P GordonElliot M Meyerowitz
Nov 28, 2009·Molecular Biology and Evolution·Nuno Pires, Liam Dolan
Apr 21, 2011·Annals of Botany·Dörte Müller, Ottoline Leyser
Feb 28, 2013·Development·Jean-Michel Davière, Patrick Achard
Nov 1, 1980·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M Koornneef, J H van der Veen
Jun 26, 2014·Development·Ram Kishor YadavG Venugopala Reddy

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
Reverse Transcription Polymerase
PCR
fluorescence microscopy
chemical treatment

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.