Bi-layered collagen nano-structured membrane prototype (collagen matrix 10826(®)) for oral soft tissue regeneration: an "in vitro" study

Clinical Oral Implants Research
Pier Francesco NociniDaniele De Santis

Abstract

To evaluate fundamental cell functions, such as adhesion, IL-6 production and proliferation of human gingival keratinocytes cultured on a newly engineered collagen matrix (CM-10826) and to assess the degree of specific biocompatibility of this new device. Primary cultures of human keratinocytes were derived "in vitro" from biopsies of independent donors. Their true epithelial origin was ensured by the expression of cytokeratin 14. Adhesion, proliferation and production of IL-6 cytokine was then measured in the presence or absence of CM-10826 activity or of its relevant components. Functional tests revealed that keratinocytes adhered to CM-10826 and up-regulated their basal IL-6 production. The type of keratinocytes used expressed cytokeratin 14. Proliferation experiments demonstrated that the best cellular response was observed in the presence of Collagen I, the main component of CM-10826. No undesired effects were observed as for keratinocyte viability, morphology or differentiation. Our results demonstrate that CM-10826 has a favourable biological effect on the "in vitro" response of gingival keratinocytes in terms of IL-6 production, cell growth and adhesion. These findings may encourage a possible use of this collagen membr...Continue Reading

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Citations

Jan 23, 2019·Minerva stomatologica·Daniele De SantisPier Francesco Nocini
Mar 12, 2019·Minerva stomatologica·Maurizio ManuelliAlessandra Lucchese
Aug 1, 2013·Clinical Implant Dentistry and Related Research·Anja ZembicDaniel Wismeijer
May 13, 2017·Minerva stomatologica·Dario BertossiAlessandro Zangani
Jan 5, 2014·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Miguel Alexandre de Araújo Nobre, Paulo Maló
Apr 2, 2014·Clinical Oral Implants Research·José Luis Calvo-GuiradoGeorgios E Romanos

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