Bias due to Preanalytical Dilution of Rodent Serum for Biochemical Analysis on the Siemens Dimension Xpand Plus

Frontiers in Veterinary Science
Jennifer L JohnsRoberta C Moorhead

Abstract

Clinical pathology testing of rodents is often challenging due to insufficient sample volume. One solution in clinical veterinary and exploratory research environments is dilution of samples prior to analysis. However, published information on the impact of preanalytical sample dilution on rodent biochemical data is incomplete. The objective of this study was to evaluate the effects of preanalytical sample dilution on biochemical analysis of mouse and rat serum samples utilizing the Siemens Dimension Xpand Plus. Rats were obtained from end of study research projects. Mice were obtained from sentinel testing programs. For both, whole blood was collectedviaterminal cardiocentesis into empty tubes and serum was harvested. Biochemical parameters were measured on fresh and thawed frozen samples run straight and at dilution factors 2-10. Dilutions were performed manually, utilizing either ultrapure water or enzyme diluent per manufacturer recommendations. All diluted samples were generated directly from the undiluted sample. Preanalytical dilution caused clinically unacceptable bias in most analytes at dilution factors four and above. Dilution-induced bias in total calcium, creatinine, total bilirubin, and uric acid was considered un...Continue Reading

References

Jan 13, 2010·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Jennifer WaldochMary Christopher
Mar 7, 2012·Veterinary Clinical Pathology·Carol KilkennyDouglas G Altman
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Dec 10, 2016·Veterinary Clinical Pathology·Florence Poitout-BelissentLila Ramaiah

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Citations

Apr 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhang ShimingMallikarjuna Rao Pichika

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