Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena

Journal of Cell Science
Masaaki IwamotoTokuko Haraguchi

Abstract

Ciliates have two functionally distinct nuclei, a somatic macronucleus (MAC) and a germline micronucleus (MIC) that develop from daughter nuclei of the last postzygotic division (PZD) during the sexual process of conjugation. Understanding this nuclear dimorphism is a central issue in ciliate biology. We show, by live-cell imaging of Tetrahymena, that biased assembly of the nuclear pore complex (NPC) occurs immediately after the last PZD, which generates anterior-posterior polarized nuclei: MAC-specific NPCs assemble in anterior presumptive MACs but not in posterior presumptive MICs. MAC-specific NPC assembly in the anterior nuclei occurs much earlier than transport of Twi1p, which is required for MAC genome rearrangement. Correlative light-electron microscopy shows that addition of new nuclear envelope (NE) precursors occurs through the formation of domains of redundant NE, where the outer double membrane contains the newly assembled NPCs. Nocodazole inhibition of the second PZD results in assembly of MAC-specific NPCs in the division-failed zygotic nuclei, leading to failure of MIC differentiation. Our findings demonstrate that NPC type switching has a crucial role in the establishment of nuclear differentiation in ciliates.

References

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Citations

Mar 11, 2016·Current Opinion in Cell Biology·Masaaki IwamotoTokuko Haraguchi
Oct 21, 2015·Communicative & Integrative Biology·Masaaki IwamotoTokuko Haraguchi
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Mar 3, 2017·PLoS Pathogens·Michael P RoutMark C Field
Jun 9, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaaki IwamotoTokuko Haraguchi

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Methods Mentioned

BETA
electron microscopy
imaging techniques
fluorescence microscopy
nuclear translocation
fluorescence
PCR

Software Mentioned

DeltaVision
CLEM

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