Biased Immunoglobulin Light Chain Gene Usage in the Shark

The Journal of Immunology : Official Journal of the American Association of Immunologists
Anna IacoangeliEllen Hsu

Abstract

This study of a large family of κ L chain clusters in nurse shark completes the characterization of its classical Ig gene content (two H chain isotypes, μ and ω, and four L chain isotypes, κ, λ, σ, and σ-2). The shark κ clusters are minigenes consisting of a simple VL-JL-CL array, where V to J recombination occurs over an ~500-bp interval, and functional clusters are widely separated by at least 100 kb. Six out of ~39 κ clusters are prerearranged in the germline (germline joined). Unlike the complex gene organization and multistep assembly process of Ig in mammals, each shark Ig rearrangement, somatic or in the germline, appears to be an independent event localized to the minigene. This study examined the expression of functional, nonproductive, and sterile transcripts of the κ clusters compared with the other three L chain isotypes. κ cluster usage was investigated in young sharks, and a skewed pattern of split gene expression was observed, one similar in functional and nonproductive rearrangements. These results show that the individual activation of the spatially distant κ clusters is nonrandom. Although both split and germline-joined κ genes are expressed, the latter are prominent in young animals and wane with age. We spec...Continue Reading

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Citations

Feb 16, 2017·Annual Review of Animal Biosciences·J E ButlerMarek Sinkora
Jan 16, 2017·Methods : a Companion to Methods in Enzymology·Paul J ConroyJames C Whisstock
Aug 2, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna IacoangeliEllen Hsu
Jan 11, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marek SinkoraKaterina Stepanova
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ellen Hsu

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