Biased short tract repair of palindromic loop mismatches in mammalian cells.

Genetics
D G TaghianJ A Nickoloff

Abstract

Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically silent 12-base palindromic loop mismatch, and/or five single-base mismatches. In wild-type cells, both loops and single-base mismatches were efficiently repaired (80-100%). When no other mismatches were present in hDNA, loops were retained with a 1.6-1.9:1 bias. However, this bias was eliminated when single-base mismatches were present, perhaps because single-base mismatches signal nick-directed repair. In the multiple marker crosses, most repair tracts were long and continuous, with preferential loss of markers in cis to proximal nicks, consistent with nicks directing most repair in this situation. However, approximately 25% of repair tracts were discontinuous as a result of loop-specific repair, or from segregation or short tract repair of single-base mismatches. In mutant cells, single-base mismatches were repaired less frequently, but the loop was still repaired efficiently and with bias toward loop retention, in...Continue Reading

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Citations

Aug 6, 2008·Human Gene Therapy·David W Russell, Roli K Hirata
Oct 3, 2003·The Journal of Biological Chemistry·Scott D McCullochGuo-Min Li
Mar 7, 2006·Mutation Research·Guliang Wang, Karen M Vasquez
Aug 4, 2005·Journal of Biomedical Science·Yi-Kuang ChuangWoei-horng Fang
Nov 5, 2003·FEBS Letters·P A Mason, R N Lightowlers
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·H MooreT D Petes
Mar 11, 2004·DNA Repair·Cheryl A MillerJac A Nickoloff
May 21, 2004·The Journal of Biological Chemistry·Yao-Ming HuangWoei-horng Fang
Jun 11, 1999·The Journal of Biological Chemistry·S E Corrette-BennettR S Lahue
Jan 6, 2009·Molecular Carcinogenesis·Guliang Wang, Karen M Vasquez

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