Bicarbonate ATP-ase in ciliary body and a theory of Diamox effect on aqueous humor formation

International Ophthalmology
E Cotlier


Bicarbonate was found to stimulate ATP breakdown by rabbit or cat ciliary body-iris homogenates. Maximum HCO3- stimulation of ATPase with Tris-Hepes buffer occured at pH 8.0. Acid pH and chloride ions in the media reduced the activity of the HCO3--stimulated ATPase. The Km for ATP was 0.55 mmolar and for HCO3-, 20 mmlar. HCO3- ATPase was not inhibited by acetazolamide added to in vitro. It is postulated that ATPase represents the linkage step of energy donor mechanism and active CT secretion in acid aqueous humors (human, cat.) or HCO3- secretion in alkaline aqueous humor (rabbit, guinea pig). Inhibition of Cl- or HCO3- secretion by acetazolamide results from decreased intracellular HCO3- levels which, in turn, reduces the stimulation of the HCO3- ATPase.


May 1, 1975·The Journal of Clinical Investigation·J A OliverP R Steinmetz
Sep 1, 1975·American Journal of Ophthalmology·G BiettiJ Pecori-Giraldi
Sep 20, 1965·Biochimica Et Biophysica Acta·D K Kasbekar, R P Durbin
Oct 12, 1971·Biochimica Et Biophysica Acta·A L BlumG Sachs
Jul 3, 1972·Biochimica Et Biophysica Acta·J J de PontS L BONTING
Sep 1, 1972·Biochimica Et Biophysica Acta·B SimonG Sachs
Oct 12, 1972·Biochimica Et Biophysica Acta·K T Izutsu, I A Siegel
Jun 1, 1971·The American Journal of Physiology·J H Schwartz, P R Steinmetz
Jan 1, 1959·American Journal of Ophthalmology·B BECKER
Jul 1, 1959·A.M.A. Archives of Ophthalmology·V E KINSEY, D V REDDY
Apr 1, 1955·American Journal of Ophthalmology·J S FRIEDENWALD
Jan 1, 1952·Proceedings of the National Academy of Sciences of the United States of America·C A Hogben


Nov 28, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Chi-ho ToChi-wai Do
Jan 1, 1995·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·C H Chiang, C H Lin
Jul 1, 2016·Taiwan Journal of Ophthalmology·Shital S PanchalDevdas D Santani

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