Bicarbonate ATP-ase in ciliary body and a theory of Diamox effect on aqueous humor formation

International Ophthalmology
E Cotlier

Abstract

Bicarbonate was found to stimulate ATP breakdown by rabbit or cat ciliary body-iris homogenates. Maximum HCO3- stimulation of ATPase with Tris-Hepes buffer occured at pH 8.0. Acid pH and chloride ions in the media reduced the activity of the HCO3--stimulated ATPase. The Km for ATP was 0.55 mmolar and for HCO3-, 20 mmlar. HCO3- ATPase was not inhibited by acetazolamide added to in vitro. It is postulated that ATPase represents the linkage step of energy donor mechanism and active CT secretion in acid aqueous humors (human, cat.) or HCO3- secretion in alkaline aqueous humor (rabbit, guinea pig). Inhibition of Cl- or HCO3- secretion by acetazolamide results from decreased intracellular HCO3- levels which, in turn, reduces the stimulation of the HCO3- ATPase.

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Citations

Nov 28, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Chi-ho ToChi-wai Do
Jan 1, 1995·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·C H Chiang, C H Lin
Jul 1, 2016·Taiwan Journal of Ophthalmology·Shital S PanchalDevdas D Santani

Related Concepts

Acetazolamide Sodium, (Sterile)
DNA-dependent ATPase
Striadyne
Metazoa
Aqueous Flare
Carbonic Acid Ions
Felis catus
Chloride Ion Level
Ciliary Body
Cavia porcellus

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