PMID: 9528661Apr 7, 1998Paper

Bicarbonate promotes a cleavage of lactone ring of dehydroascorbate

Biochimica Et Biophysica Acta
I KoshiishiT Imanari

Abstract

The half-life of dehydroascorbate (DHA) in human plasma is only a few minutes. This DHA disappearance is caused by a cleavage of lactone ring. Similarly, when DHA was incubated in Dulbecco's modified Eagle's medium (D-MEM), which stood in atmosphere of 5% CO2-95% air, the rapid transformation of DHA into 2,3-diketogulonate (2,3-DKG) is also observed. These observations suggest that both human plasma and D-MEM contain a common component, which promotes the hydrolysis of DHA. In the present study, this component was identified to be bicarbonate which acts as a general base catalyst. Direct evidence for this mechanism was obtained as follows: (1) significant hydrolysis of DHA in the bicarbonate-free D-MEM (pH 7.40) was not observed; (2) hydrolysis of DHA in Tris-HCl buffer at constant pH (7.4) increases with increasing bicarbonate concentration; and (3) significant hydrolysis of DHA in the decarbonated ultrafiltrate of plasma was not observed. These results suggest that DHA hydrolysis may be controlled by the variation of CO2 pressure in circulating blood.

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Citations

Apr 3, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·P M Abuja, R Albertini
Jun 23, 2011·Journal of the American Chemical Society·Sarah E BohndiekKevin M Brindle
Jan 16, 2007·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Jul 23, 2014·Free Radical Biology & Medicine·Darius J R Lane, Des R Richardson
Jan 10, 2012·Phytochemistry·Harriet T Parsons, Stephen C Fry
Oct 21, 2004·Free Radical Research·Patric J JanssonTommy Nordström
Jun 5, 2007·Journal of Biochemical and Biophysical Methods·Luka Wechtersbach, Blaz Cigić
Sep 11, 2002·FEBS Letters·John X Wilson
Nov 19, 2002·The Journal of Biological Chemistry·Francisco J NualartJuan Carlos Vera
Feb 21, 2002·Biochimica Et Biophysica Acta·Oreste Arrigoni, Mario C De Tullio
Nov 14, 1998·Biochimica Et Biophysica Acta·I KoshiishiT Imanari
Jul 25, 2000·Journal of Chromatography. a·J C Deutsch
Apr 12, 2019·The Science of the Total Environment·Linda PasteroAlessandro Pavese

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