Bicistronic and two-gene retroviral vectors for using MDR1 as a selectable marker and a therapeutic gene

Virology
M Z MetzS E Kane

Abstract

We describe a series of two-gene and bicistronic retroviral vectors that use the human MDR1 gene as a selectable marker for the overexpression of a second heterologous gene in transduced cells. The vectors use Harvey murine sarcoma virus sequences for viral expression and packaging functions and include sites for cloning foreign genes of interest under the control of either an internal promoter (two-gene vectors) or an internal ribosome entry site (bicistronic vectors). To characterize these vectors, we used neo as a reporter gene for foreign gene expression and as an independently selectable marker for comparison with MDR1. Each of the vector constructions supported high-titer retrovirus production and transduction of mouse and human cell lines. Using MDR1-neo virus supernatants in parallel titering assays, we found that titers based on colchicine resistance were 10- to 20-fold lower than titers based on G418 resistance, suggesting that MDR1 is a more stringent selectable marker than neo in NIH 3T3 and KB-3-1 cell lines. Whereas neo gene expression with the two-gene vectors was subject to host-specific limitations on internal promoter activity, the bicistronic vectors were highly active in three cell lines tested. In K562 cell...Continue Reading

Citations

Jan 1, 1997·Stem Cells·T LichtI Pastan
Aug 1, 1997·Nature Biotechnology·A ParikhF P Guengerich
Sep 1, 1996·Cancer Metastasis Reviews·J A RaffertyL J Fairbairn
Nov 3, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M J KelnerR Taetle
Mar 1, 1997·Antimicrobial Agents and Chemotherapy·C BethuneR J Ho

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