Bicistronic expression plasmid encoding allergen and anti-IgE single chain variable fragment antibody as a novel DNA vaccine for allergy therapy and prevention

Medical Hypotheses
Nariman Aghaei Bandbon BalengaRichard Weiss

Abstract

Several approaches have been applied in order to alleviate the difficulties allergic patients are suffering from. Among them DNA vaccination and anti-IgE antibody have shown promising results. Herewith, a combination of both strategies is proposed to minimize IgE production while inducing high levels of blocking IgG and strong Th1 immune responses. A bicistronic expression plasmid including an internal ribosomal entry site (IRES) can express both, allergen and a single chain variable fragment (scFv) antibody against human IgE within antigen presenting cells (APCs) including B cells. Presentation of allergen derived peptides via MHC I and MHC II stimulates specific Th1 responses resulting in high levels of IFN-gamma and IgG. Anti-IgE scFv antibody binds to newly synthesized IgE molecules within B cell cytoplasm and also to free serum IgE, thereby inhibiting attachment of IgE to its receptors on basophils and mast cells. Also, IgE-anti-IgE complex functions as blocking antibody and neutralizes allergens entering the body. Additionally, anti-IgE scFv antibody binds to membrane bound IgE (mIgE) on B cells and interferes with IgE expression. Using assays, such as enzyme linked immunosorbent assay (ELISA), IgG and IgE production in r...Continue Reading

References

May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·E RazD A Carson
Dec 16, 1998·Current Opinion in Pediatrics·S E Crater, T A Platts-Mills
Feb 5, 2000·Nature Biotechnology·T W Chang
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Arnulf HartlJosef Thalhamer
Oct 27, 2005·Current Drug Targets. Inflammation and Allergy·Richard WeissJosef Thalhamer

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