PMID: 6538201Mar 1, 1984Paper

Bidirectional polymerization of G-actin on the human erythrocyte membrane

The Journal of Cell Biology
S TsukitaH Ishikawa

Abstract

The directional polymerization of actin on the erythrocyte membrane has been examined at various concentrations of G-actin by thin-section electron microscopy. For this purpose, a new experimental system using single-layered erythrocyte membranes with the cytoplasmic surfaces freely exposed was developed. The preformed actin filaments did not bind with the cytoplasmic surface of the erythrocyte membranes. When the erythrocyte membranes were incubated at low concentrations (0.3 and 0.5 microM) of G-actin, greater than 80% of polymerized actin filaments pointed toward the membranes mainly in an end-on fashion, as judged by arrowhead formation with heavy meromyosin. At higher concentrations (2 and 4 microM) of G-actin, about half of the polymerized actin filaments were directed with arrowheads pointing toward the membranes, while the rest of the filaments showed the opposite polarity pointing away from the membranes. The majority of polymerized actin filaments formed loops at the points of attachment to the membranes. In contrast, when G-actin (2 and 4 microM) in the presence of cytochalasin B was polymerized into filaments, approximately 70% showed the polarity pointing away from the membrane mainly in an end-on fashion. To check...Continue Reading

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Citations

Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T J Byers, D Branton
Jan 1, 1985·The Journal of Cell Biology·S Ishiwata, T Funatsu
Jan 1, 1986·The Journal of Cell Biology·E M Bonder, M S Mooseker
Mar 1, 1987·The Journal of Cell Biology·S C LiuJ Palek
Sep 1, 1984·The Journal of Cell Biology·B W ShenT L Steck
Jan 1, 1988·CRC Critical Reviews in Biochemistry·S R GoodmanI S Zagon
Jan 1, 1986·Journal of Cellular Biochemistry·V M Fowler
Apr 5, 1996·The Journal of Biological Chemistry·P A KuhlmanV M Fowler
Oct 5, 1986·Journal of Molecular Biology·J C PinderW B Gratzer

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