PMID: 7545916Mar 11, 1994Paper

Bidirectional transcription from the human immunoglobulin VH6 gene promoter

Nucleic Acids Research
Z Sun, G R Kitchingman

Abstract

The human immunoglobulin (Ig) heavy chain VH6 gene promoter contains an imperfect octamer (AgGCAAAT) and is not dependent on the Ig heavy chain enhancer for activity; reporter constructs containing this promoter are very active in non-B cells. In experiments designed to characterize regions upstream of the transcriptional start site that are important for promoter function, we produced a series of deletion constructs, including one containing sequences between -74 and -146. Surprisingly, this fragment had promoter activity in both orientations. Inspection of the VH6 promoter sequence indicated that there was a possible TATA box in the proper orientation upstream of the imperfect octamer. The -74 to -146 fragment functioned as a promoter in the reverse orientation in three B cell lines and in non-B (HeLa) cells, with a much higher level of activity seen in the HeLa cells. To determine if the promoter could work in both directions simultaneously, reporter genes were positioned up- and downstream of a VH6 promoter fragment. Reporter gene activity was found for both genes in B cells and HeLa cells. Using a reverse transcriptase-polymerase chain reaction procedure (RT-PCR), we found a transcript corresponding to sequences upstream o...Continue Reading

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Citations

May 18, 2010·Cellular & Molecular Immunology·Xiaohui ZhuXiaoyan Qiu
Dec 11, 2014·Cancer Cell International·Lina WuXiaoyan Qiu
May 5, 2020·Nucleic Acids Research·Ivana MikocziovaLudvig M Sollid
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Colette M JohnstonAnne E Corcoran
Jun 28, 2021·Genes and Immunity·Ivana MikocziovaLudvig M Sollid

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