Apr 13, 2016

Bidirectional transcription marks accessible chromatin and is not specific to enhancers

BioRxiv : the Preprint Server for Biology
Robert S YoungMartin S Taylor

Abstract

Bidirectional transcription initiating at enhancers has been proposed to represent the signature of enhancer activity. Here we show that bidirectional transcription is a pervasive feature of all forms of accessible chromatin, including enhancers, promoters, CTCF-bound sites and other DNase hypersensitive regions. Transcription is less predictive for enhancer activity than epigenetic modifications such as H3K4me1 or the accessibility of DNA when measured in both enhancer assays and at endogenous loci. Bidirectional transcription initiation from accessible chromatin is therefore not sufficient for, nor specific to, enhancer activity. The stability of enhancer initiated transcripts does not influence measures of enhancer activity and we cannot detect any evidence of purifying selection on the resulting enhancer RNAs within the human population. Our results suggest that transcription initiating at enhancers is frequently a by-product of promiscuous RNA polymerase activity at accessible chromatin, and may not generally play a functional role in enhancer activity.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Deoxyribonuclease I
DNA-Directed RNA Polymerase
Transcription, Genetic
Transcription Initiation
Promoter
Study of Epigenetics
Site
RNA Polymerase Activity
Chromatin Location
CTCF gene

About this Paper

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.