PMID: 8610154Apr 2, 1996Paper

Bidirectional uncoating of the genomic RNA of a helical virus

Proceedings of the National Academy of Sciences of the United States of America
X Wu, J G Shaw

Abstract

An essential step in the initiation of a virus infection is the release of the viral genome from the other constituents of the virus particle, a process referred to as uncoating. We have used reverse transcription and polymerase chain reaction amplification procedures to determine the rate and direction of in vivo uncoating of the rod-shaped tobacco mosaic virus. The virus particles contain a single 6.4-kb RNA molecule that lies between successive turns of a helical arrangement of coat protein subunits. When the particles are introduced into plant cells, the subunits are removed via a bidirectional uncoating mechanism. Within 2-3 min, the part of the viral RNA from the 5' end to a position >70% toward the 3' end has been freed of coat protein subunits. This is followed by removal of subunits from the 3' end of the RNA and sequential uncoating of the RNA in a 3'-to-5' direction. An internal region of the viral RNA is the final part to be uncoated. Progeny virus particles are detected in the cells 35-40 min after inoculation.

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G LebeurierK E Richards
Jun 1, 1985·The Journal of General Virology·T M Wilson
Jan 1, 1971·Nature·D K StewartM T Ragab
Jan 13, 1971·Nature: New Biology·P J Butler, A Klug
Apr 1, 1994·Virology·X WuJ G Shaw

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Citations

Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B D Harrison, T M Wilson
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G Shaw
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K W Buck
Jul 18, 2000·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·Y Okada
Jul 31, 2002·Annual Review of Phytopathology·James N Culver
Jun 7, 2014·Annual Review of Phytopathology·Pooja Saxena, George P Lomonossoff
Sep 29, 2004·Biophysical Chemistry·Jose L R SantosCarlos F S Bonafe
Jun 28, 2012·Biophysical Journal·Daniela J KraftPaul van der Schoot
Jul 17, 2020·Frontiers in Plant Science·Ekaterina V SheshukovaTatiana V Komarova

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