Bienzyme amperometric biosensor using gold nanoparticle-modified electrodes for the determination of inulin in foods

Analytical Biochemistry
Javier MansoJosé M Pingarrón

Abstract

A biosensor design involving coimmobilization of fructose dehydrogenase (FDH) and inulinase (INU) on a gold nanoparticle-cysteamine (Cyst) self-assembled monolayer (SAM)-modified gold electrode (Au(coll)-Cyst-AuE), for the determination of the carbohydrate inulin in foodstuffs, is reported. Tetrathiafulvalene (TTF), used as the mediator, was also coimmobilized by crosslinking with glutaraldehyde. INU catalyzes the hydrolysis of inulin, forming fructose that is detected through the fructose dehydrogenase system by the electrochemical oxidation of TTF at the bioelectrode. The variables involved in the preparation and performance of both the single enzyme FDH biosensor and the bienzyme inulin biosensor were optimized. The FDH-Au(coll)-Cyst-AuE biosensor exhibited rapid and sensitive response to fructose, allowing the obtention of improved analytical characteristics for the determination of fructose with respect to other FDH electrochemical biosensors. Moreover, the lifetime of this biosensor was 35 days. The bienzyme INU/FDH-Au(coll)-Cyst-AuE biosensor provided a calibration plot for inulin in the (5-100)x10(-6) M linear range, with a detection limit of 6.6 x 10(-7) mol L(-1). One single bienzyme biosensor responded within the con...Continue Reading

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Citations

Nov 11, 2009·Bulletin of Experimental Biology and Medicine·T A KovalevaS S Bogdanova
Jul 10, 2009·Chemical Society Reviews·Elodie Boisselier, Didier Astruc
Jan 16, 2014·Bioanalysis·Franco MazzeiCristina Tortolini
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Jan 16, 2015·Biosensors & Bioelectronics·Sang-Hee LeeYang-Hoon Kim
Jul 12, 2016·Biosensors & Bioelectronics·Paulo A Raymundo-PereiraOsvaldo N Oliveira
Nov 7, 2008·Phytochemical Analysis : PCA
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