Bifidobacterium breve UCC2003 Induces a Distinct Global Transcriptomic Program in Neonatal Murine Intestinal Epithelial Cells.

IScience
R. KiuL J Hall

Abstract

The underlying health-driving mechanisms of Bifidobacterium during early life are not well understood, particularly how this microbiota member may modulate the intestinal barrier via programming of intestinal epithelial cells (IECs). We investigated the impact of Bifidobacterium breve UCC2003 on the transcriptome of neonatal murine IECs. Small IECs from two-week-old neonatal mice administered B. breve UCC2003 or PBS (control) were subjected to global RNA sequencing, and differentially expressed genes, pathways, and affected cell types were determined. We observed extensive regulation of the IEC transcriptome with ∼4,000 genes significantly up-regulated, including key genes linked with epithelial barrier function. Enrichment of cell differentiation pathways was observed, along with an overrepresentation of stem cell marker genes, indicating an increase in the regenerative potential of the epithelial layer. In conclusion, B. breve UCC2003 plays a central role in driving intestinal epithelium homeostatic development during early life and suggests future avenues for next-stage clinical studies.

References

Jan 1, 1991·Annals of the New York Academy of Sciences·D F MosherE L Barry
Apr 1, 1997·Digestive Diseases and Sciences·C FavierJ Mizon
Jul 25, 2000·Postgraduate Medical Journal·T ShiraziC S Probert
Jun 23, 2004·PLoS Biology·Jun QinEdward F Plow
Jun 15, 2006·World Journal of Gastroenterology : WJG·Christian-U RiedelStephanie Blum
Jul 13, 2006·Gastroenterology·Maria Van der SluisAlexandra W C Einerhand
Apr 9, 2008·Cell Research·Roel Nusse
Sep 13, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Julia B EwaschukKaren L Madsen
Dec 17, 2008·Scandinavian Journal of Gastroenterology·Hans HildebrandScott M Montgomery
Jan 2, 2009·Journal of Cell Science·David S Harburger, David A Calderwood
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·Katherine R Groschwitz, Simon P Hogan
Jul 28, 2009·Current Opinion in Pharmacology·Karen L Edelblum, Jerrold R Turner
Aug 22, 2009·Experimental & Molecular Medicine·Yangsik Jeong, David J Mangelsdorf
May 12, 2010·World Journal of Gastroenterology : WJG·Chang LiuXiao-Kui Guo
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Maria G Dominguez-BelloRob Knight
Oct 14, 2010·The American Journal of Gastroenterology·Souradet Y ShawCharles N Bernstein
Jan 25, 2011·International Journal of Food Microbiology·David PhilippeChristian U Riedel
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mary O'Connell MotherwayDouwe van Sinderen
Nov 19, 2013·Applied and Environmental Microbiology·Francesca TurroniMarco Ventura
Nov 20, 2013·Pediatrics·Susan E JacobsUNKNOWN ProPrems Study Group
Jan 1, 2012·Pharmaceuticals·Katsunaka MikamiHidenori Takahashi
Sep 16, 2014·Seminars in Cell & Developmental Biology·Susanne M KrugMichael Fromm
Sep 25, 2014·Frontiers in Immunology·Marie-Claire ArrietaBrett Finlay
Jan 7, 2015·World Journal of Gastroenterology : WJG·Li ZuoDing-Hua Yang
Mar 10, 2015·International Journal of Hematology·Maria Rosaria ImperatoConstanze Bonifer
Mar 18, 2015·Physiological Reports·Chen-Yu HsiehSatoshi Tsuneda
Oct 31, 2016·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·Benjamin James BaucellsJosep Figueras Aloy
Nov 17, 2017·Nature·Adam L HaberAviv Regev

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Citations

Jun 1, 2021·Frontiers in Immunology·Cristina KalbermatterStephanie C Ganal-Vonarburg

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Datasets Mentioned

BETA
PRJEB36661

Methods Mentioned

BETA
IEC
IECs
RNA-seq
Amplicon Sequencing
PCA
ubiquitination
biopsies

Software Mentioned

PANTHER GO - Slim
DoRothEA
PANTHER

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