Bifunctional Super-resolution Imaging Probe with Acidity-Independent Lysosome-Retention Mechanism

Analytical Chemistry
Zhongwei XueShoufa Han

Abstract

Spatiotemporal imaging is of enormous use to explore organelle biology, necessitating organelle-tracing techniques reliable in varied cell stress. We herein reported lysosomal imaging using rhodamine-X-integrated sialic acid (ROXSA), which is stably maintained in lysosomes irrespective of lysosomal pH changes. Exhibiting bright fluorescence and superior photostability, ROXSA enables 120 h continual tracking of fusion/fission of lysosomes and mitochondrion-lysosome interaction in mitophagy. Relative to conventional acidotropic probes prone to dissipation from stressed lysosomes, ROXSA offers a new route for long-term tracking of stressed lysosomes relevant to diverse pathological conditions.

References

Feb 1, 1979·Archives of Neurology·P AulaI Yamashina
Oct 10, 1997·Nucleic Acids Research·C Li, R M Evans
Jul 10, 1999·Pflügers Archiv : European journal of physiology·E A HardingD I Cook
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Ros-BaroM Camps
Jun 25, 2002·Methods in Enzymology·Eric J Smart, Richard G W Anderson
Mar 5, 2004·Chembiochem : a European Journal of Chemical Biology·Sarah J LuchanskyCarolyn R Bertozzi
Jul 3, 2004·Science·Jane StinchcombeGillian M Griffiths
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ikuhiko NakaseShiroh Futaki
Apr 19, 2005·Cancer Research·Nicole Fehrenbacher, Marja Jäättelä
Aug 23, 2005·Experimental Cell Research·Jameel InalJürg A Schifferli
Oct 22, 2005·Nature Reviews. Cancer·Guido Kroemer, Marja Jäättelä
Jan 13, 2006·Nature Chemical Biology·Shoufa HanJames C Paulson
Feb 14, 2006·Nature Chemical Biology·Srinivasa-Gopalan SampathkumarKevin J Yarema
Jul 21, 2007·Angewandte Chemie·J FöllingS W Hell
Feb 28, 2009·Pediatric Research·Aviram Kogot-LevinGideon Bach
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Stephen W G Tait, Douglas R Green
Aug 11, 2010·Mitochondrion·Urška Repnik, Boris Turk
Oct 30, 2012·Nature Methods·Joshua C VaughanXiaowei Zhuang
Apr 24, 2013·Nature Reviews. Molecular Cell Biology·Carmine SettembreAndrea Ballabio
Sep 18, 2013·Organic Letters·Guoping LiHua Jiang
Jun 24, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Agnieszka Pierzyńska-MachJurek W Dobrucki
Aug 25, 2015·Cell Metabolism·Francesc BaixauliMaria Mittelbrunn
Nov 10, 2015·Neurobiology of Disease·Sophia Von StockumElena Ziviani
Dec 22, 2015·Advanced Healthcare Materials·Chris Wai Tung LeungBen Zhong Tang
Sep 14, 2016·The Journal of Cell Biology·Chun-Yan Lim, Roberto Zoncu
Feb 14, 2017·Trends in Neurosciences·Jesse S KerrEvandro F Fang
May 27, 2017·Circulation Research·José Manuel Bravo-San PedroLorenzo Galluzzi
Feb 16, 2018·Journal of the American Chemical Society·Yuting SunXing Chen

❮ Previous
Next ❯

Citations

Sep 28, 2018·Chemical Communications : Chem Comm·Rui ZhuShoufa Han
Jan 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuo GuoZhaohui Li
Oct 28, 2020·Chemical Communications : Chem Comm·Jintao ZhaoJianguo Fang
Aug 11, 2020·ACS Omega·Enkang ZhangXinhui Su
Nov 3, 2020·ACS Omega·Aditya YadavChayan K Nandi
Jul 1, 2020·The Journal of Physical Chemistry Letters·Aditya YadavChayan K Nandi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Biotechnology
Joshua C Vaughan, Xiaowei Zhuang
Nature Methods
Regan P Moore, Wesley R Legant
Rinsho byori. The Japanese journal of clinical pathology
K Tsurumi
© 2022 Meta ULC. All rights reserved