Bilateral activation of STAT3 by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and its nuclear translocation in primary sensory neurons following unilateral sciatic nerve injury

Histochemistry and Cell Biology
Petr DubovýMarek Joukal

Abstract

Unilateral sciatic nerve compression (SNC) or complete sciatic nerve transection (CSNT), both varying degrees of nerve injury, induced activation of STAT3 bilaterally in the dorsal root ganglia (DRG) neurons of lumbar (L4-L5) as well as cervical (C6-C8) spinal cord segments. STAT3 activation was by phosphorylation at the tyrosine-705 (Y705) and serine-727 (S727) positions and was followed by their nuclear translocation. This is the first evidence of STAT3(S727) activation together with the well-known activation of STAT3(Y705) in primary sensory neurons upon peripheral nerve injury. Bilateral activation of STAT3 in DRG neurons of spinal segments anatomically both associated as well as non-associated with the injured nerve indicates diffusion of STAT3 activation inducers along the spinal cord. Increased levels of IL-6 protein in the CSF following nerve injury as well as activation and nuclear translocation of STAT3 in DRG after intrathecal injection of IL-6 shows that this cytokine, released into the subarachnoid space can penetrate the DRG to activate STAT3. Previous results on increased bilateral IL-6 synthesis and the present manifestation of STAT3 activation in remote DRG following unilateral sciatic nerve injury may reflect ...Continue Reading

References

Oct 17, 1980·European Journal of Pharmacology·J L Hylden, G L Wilcox
Jun 13, 2000·Oncogene·T Decker, P Kovarik
Jun 1, 2002·Science·David S Aaronson, Curt M Horvath
Jul 11, 2002·Histochemistry and Cell Biology·Petr DubovýIvana Svízenská
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Feb 18, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jin QiuStephen W N Thompson
Jan 13, 2006·The Journal of Cell Biology·Dominic Chi Hiung NgXinmin Cao
Jan 13, 2006·The Journal of Biological Chemistry·Mehul ShahPravin B Sehgal
Apr 14, 2006·The Journal of Biological Chemistry·Yu Pong NgNancy Y Ip
Sep 15, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tizong MiaoPeter M Richardson
Oct 31, 2006·Biochemical and Biophysical Research Communications·Vaclav BrazdaBorivoj Vojtesek
Jul 2, 2008·Seminars in Cell & Developmental Biology·James Castelli-Gair Hombría, Sol Sotillos
Mar 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Florence M BareyreMartin Kerschensteiner
May 31, 2011·Experimental Cell Research·Christopher L GermanCharles L Howe
Dec 6, 2011·European Journal of Cell Biology·René EulenfeldFred Schaper
Jan 12, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryohei WakaharaKoichi Nakajima
Jan 31, 2012·Neuron·Céline S NicolasGraham L Collingridge
Mar 1, 2012·Frontiers in Molecular Neuroscience·Smriti Patodia, Gennadij Raivich
Nov 9, 2012·International Journal of Biological Sciences·María ErtaJuan Hidalgo
Nov 9, 2012·International Journal of Biological Sciences·Masaaki Murakami, Toshio Hirano
Jun 19, 2013·Journal of Neuropathology and Experimental Neurology·Annina B SchmidElspeth M McLachlan
Jul 9, 2013·Molecular and Cellular Neurosciences·Michael J Pellegrino, Beth A Habecker
Feb 13, 2014·Cardiovascular Research·Arash HaghikiaDenise Hilfiker-Kleiner
Feb 5, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Marek JoukalPetr Dubový
Jun 29, 2016·Cytokine·Jan PencikLukas Kenner
Dec 25, 2016·Journal of Molecular Neuroscience : MN·Hsueh-Ling ChangKuang-I Cheng

❮ Previous
Next ❯

Citations

Jan 30, 2020·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Dec 14, 2018·Frontiers in Cellular Neuroscience·Andrea KorimováPetr Dubový
Jun 18, 2020·Cancers·Hae-Seul ChoiKwang-Hyun Baek
Nov 17, 2020·Frontiers in Oncology·Donglu WuHaoming Luo

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
electrophoresis

Software Mentioned

STATISTICA
Elements
NIS

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.