PMID: 8967344Apr 1, 1996Paper

Bilateral ureteral obstruction downregulates expression of vasopressin-sensitive AQP-2 water channel in rat kidney

The American Journal of Physiology
Jørgen FrøkiaerSøren Nielsen

Abstract

Polyuria after release of bilateral ureteral obstruction (BUO) is frequently seen in patients with urological disorders. In this study, we examined the effect of BUO and release of BUO on the expression of the vasopressin-regulated water channel aquaporin-2 (AQP-2) in rat kidney. Ureters were obstructed for 24 h in all experiments, and BUO was either not released or released for 24 or 48 h or 7 days. Each group of experimental rats were matched with sham-operated controls. One kidney was used for membrane fractionation and immunoblotting, whereas the contralateral was fixed for immunocytochemistry. Immunoblotting demonstrated a significant reduction in AQP-2 expression in inner medullar during 24 h of BUO to 26 +/- 8% (P < 0.001). Release of BUO was associated with immediate onset of a predominant osmotic-dependent polyuria. Forty-eight hours after release of BUO, the reduction in AQP-2 expression persisted (19 +/- 8%, P < 0.001), concurrent with a marked nonosmotic postobstructive polyuria, as determined by a significant reduction in free-water clearance (-50 +/- 7 vs. -85 +/- 10 microliters.min-1.kg-1, P < 0.05). Immunofluorescence and immunoelectron microscopy confirmed the reduced levels of AQP-2 in collecting duct principa...Continue Reading

Associated Clinical Trials

Citations

Feb 9, 2000·American Journal of Physiology. Renal Physiology·T E JonassenS Nielsen
Jan 9, 2003·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
Dec 17, 2009·American Journal of Physiology. Renal Physiology·Yue ZhangBellamkonda K Kishore
Jan 5, 2002·Physiological Reviews·Søren NielsenMark A Knepper
Jul 17, 2003·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
Nov 30, 2000·American Journal of Physiology. Renal Physiology·T E JonassenS Nielsen
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
Jan 19, 2007·American Journal of Physiology. Renal Physiology·Rikke NørregaardJørgen Frøkiaer
Jun 8, 2007·American Journal of Physiology. Renal Physiology·Robert A FentonJeppe Praetorius
May 30, 2008·American Journal of Physiology. Renal Physiology·Guixian WangJørgen Frøkiaer
Apr 29, 2011·American Journal of Physiology. Renal Physiology·Lene StødkildeJørgen Frøkiær
Jan 29, 2010·American Journal of Physiology. Renal Physiology·Anja M JensenJørgen Frøkiaer
Apr 25, 2014·American Journal of Physiology. Renal Physiology·Lene StødkildeJørgen Frøkiaer
Feb 26, 2004·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
May 4, 2007·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
Sep 29, 2005·American Journal of Physiology. Renal Physiology·Chunling LiJørgen Frøkiaer
May 23, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Joris H Robben, Peter M T Deen
Oct 27, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Guixian WangJørgen Frøkiær
Nov 30, 2006·American Journal of Physiology. Renal Physiology·Anni Eskild-JensenJørgen Frøkiaer
Feb 5, 2010·American Journal of Physiology. Renal Physiology·Erin BaggaleyDavid Marples
Oct 31, 2008·American Journal of Physiology. Renal Physiology·Anja M JensenJørgen Frøkiaer
Jul 15, 2016·American Journal of Physiology. Renal Physiology·Ying SunZhanjun Jia
Nov 19, 2019·American Journal of Physiology. Renal Physiology·Poorichaya SomparnSookkasem Khositseth
Feb 7, 2017·BioMed Research International·Anabel Brandoni, Adriana M Torres
Apr 21, 2005·American Journal of Physiology. Renal Physiology·Rikke NørregaardJørgen Frøkiaer
Jul 11, 2006·American Journal of Physiology. Renal Physiology·Joris H RobbenPeter M T Deen
Dec 15, 2020·International Journal of Nephrology·Jonathan S Chávez-IñiguezGuillermo García-García
May 29, 2021·Canadian Journal of Physiology and Pharmacology·Anabel Brandoni, Adriana M Torres

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