Bilayer polymeric nanocapsules: A formulation approach for a thermostable and adjuvanted E. coli antigen vaccine

Journal of Controlled Release : Official Journal of the Controlled Release Society
José Crecente-CampoM J Alonso

Abstract

One of the strategies used to improve the immunogenicity of purified protein antigens has relied on their association with synthetic nanocarriers, which, in general, have functioned as simple antigen containers. Here, we present a more advanced strategy based on the design of an antigen nanocarrier at the molecular level. The nanocarrier is composed of a vitamin E oily core, surrounded by two layers: a first layer of chitosan and a second of dextran sulphate. The selected antigen, IutA protein from Escherichia coli, was harboured between the two polymeric layers. The final bilayer nanocapsules had a nanometric size (≈ 200 nm), a negative zeta potential (< -40 mV) and a good antigen association efficiency (≈ 70%). The bilayer architecture led to an improvement on the formulation stability and the controlled release of the associated antigen. Remarkably, after being administered to mice, bilayer nanocapsules elicited higher IgG levels than those obtained with antigen precipitated with Alum. Moreover, freeze-dried nanocapsules were stable at room temperature for, at least, 3 months. These promising data, in addition to their contribution to the development of an uropathogenic E. coli vaccine, has allowed us to validate these novel...Continue Reading

Citations

Nov 8, 2019·Nanomedicine·José Crecente-CampoMaría J Alonso
Feb 11, 2020·Drug Delivery and Translational Research·Tamara G DacobaJosé Crecente-Campo
Jan 27, 2019·Bioengineering & Translational Medicine·José Crecente-Campo, María José Alonso
Aug 28, 2020·The Journal of Clinical Investigation·Hongzhao LiMa Luo
Jul 22, 2019·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Sonia Reimondez-TroitiñoMaría de la Fuente
Nov 17, 2020·International Journal of Nanomedicine·Alicja KarabaszKrzysztof Szczepanowicz
Apr 3, 2021·Drug Delivery and Translational Research·Ana Sara CordeiroMaría José Alonso
May 7, 2021·Frontiers in Cellular and Infection Microbiology·Sofía V SánchezJavier O Morales
Mar 17, 2021·Expert Review of Vaccines·Yizhi Qi, Christopher B Fox
Jul 28, 2021·Advanced Drug Delivery Reviews·Matilde Durán-LobatoMaría José Alonso
Aug 8, 2021·Nanomaterials·Alba Pensado-LópezFernando Torres Andón

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