Bile acid synthesis in the isolated, perfused rabbit liver

The Journal of Clinical Investigation
E H MosbachJ Mongelli

Abstract

These experiments were carried out to demonstrate the usefulness of the perfused rabbit liver for studies of bile acid metabolism, and to determine the rate-limiting enzyme of bile acid synthesis. Rabbits were fed a semisynthetic diet, with or without the addition of 1% cholestyramine, under controlled conditions. At the end of 2-5 wk, the livers were removed and perfused for 2.5 hr employing various (14)C-labeled precursors to measure de novo cholic acid synthesis. The livers were then analyzed for cholesterol, and the bile collected during the perfusion was analyzed for cholesterol and bile acids. Control bile contained, on the average, 0.34 mg of glycocholate, 7.4 mg of glycodeoxycholate, and 0.06 mg of cholesterol. After cholestyramine treatment of the donor rabbits, the bile contained 3.3 mg of glycocholate, 3.7 mg of glycodeoxycholate, and 0.05 mg of cholesterol. It was assumed that in cholestyramine-treated animals the enterohepatic circulation of the bile acids had been interrupted sufficiently to release the feedback inhibition of the rate-controlling enzyme of bile acid synthesis. Therefore, a given precursor should be incorporated into bile acids at a more rapid rate in livers of cholestyramine-treated animals, provi...Continue Reading

References

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Citations

Oct 1, 1974·The American Journal of Digestive Diseases·C D FischerE H Mosbach
Oct 1, 1974·The American Journal of Digestive Diseases·E H Mosbach, G Salen
Dec 15, 1973·Molecular and Cellular Biochemistry·K Decker, C Barth
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Related Concepts

Acetic Acids
Biliary Sludge
Bile Salts
Carbon Isotopes
Chemistry, Organic
Epicholesterol
MK-135
Feedback - System Communication
Hepatectomy
Hydroxylation

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