Bile duct-bound growth of precursor cells of preneoplastic foci inducible in the initiation stage of rat chemical hepatocarcinogenesis by 2-acetylaminofluorene

Japanese Journal of Clinical Oncology
Kimihiko SatohMakoto Hayakari

Abstract

We previously detected precursor cell populations of preneoplastic foci, GST-P(+)/GGT(-) and GST-P(+)/GGT(+) minifoci, in rat liver in the initiation stage of chemical hepatocarcinogenesis, where GST-P and GGT represent glutathione S-transferase P-form and gamma-glutamyltranspeptidase, respectively. Sprague-Dawley male rats were fed a basal diet containing 2-acetylaminofluorene (0.02%) over 16 weeks. Precursor cells were detected by our sensitive staining method for GGT activity and immunocytochemical staining for GST-P. GST-P(+)/GGT(-) single cells were overproduced maximally in the animal liver after the 6 weeks followed by a gradual growth of GST-P(+)/GGT(-) and GST-P(+)/GGT(+) minifoci, which were bound to bile ducts and ductules. GGT was expressed within GST-P(+) minifoci gradually with time forming GGT(+) lane-like structures. The bile duct binding and lane-like structure formation were prominent especially when minifoci-bearing rats were subjected to two-thirds partial hepatectomy. A variety of precursor minifoci were noted to be selectively bound to bile ducts and ductules in rat liver, which may be of physiologic significance in excretion of carcinogens during initiation.

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Jan 1, 1978·Annual Review of Pharmacology and Toxicology·W G Levine
Jun 15, 1990·Biochemical Pharmacology·E Farber
Jan 1, 1989·Advances in Cancer Research·K Sato
Sep 11, 1985·Nucleic Acids Research·Y SuguokaM Muramatsu
Mar 1, 1987·Carcinogenesis·M A MooreK Sato
Aug 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M RutenburgA M Seligman
Jan 1, 1980·CRC Critical Reviews in Clinical Laboratory Sciences·D M Goldberg
Jan 1, 1980·Advances in Cancer Research·E Farber, R Cameron
Jul 5, 2003·Scientific American·W Wayt Gibbs
Feb 25, 2005·International Journal of Cancer. Journal International Du Cancer·Kimihiko SatohIchiro Hatayama

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