PMID: 39496May 1, 1979

Bile salt 3 alpha- and 12 alpha-hydroxysteroid dehydrogenases from Eubacterium lentum and related organisms

Applied and Environmental Microbiology
I A MacdonaldL V Holdeman

Abstract

Thirty-two strains of Eubacterium lentum and phenotypically similar anaerobic gram-positive bacilli were screened for intracellular bile salt 3alpha- and 12alpha-hydroxysteroid dehydrogenase (HSDHase) activities. These organisms were categorized into four groups: (A) those containing 12alpha-HSDHase only (10 strains), (B) those containing 3alpha- and 12alpha-HSDHase (13 strains), (C) those containing 3alpha-HSDHase only (2 strains), and (D) those devoid of any measurable HSDHase activity (7 strains). Of the respective four groups, 9/10, 13/13, 0/2, and 0/7 were like the neotype strain of E. lentum (ATCC 25559) in that they produced H(2)S in a triple sugar iron agar butt, reduced nitrate to nitrite, and weakly decomposed hydrogen peroxide. The other strains were variable for nitrate reduction and activity on hydrogen peroxide, but all the organisms in the first three categories (with one exception) were H(2)S producers (triple sugar iron agar butt) and all (with one exception) were designated E. lentum, whereas the organisms of category B were non-H(2)S producers (triple sugar iron agar butt). Five of these seven were not stimulated by arginine and are designated "phenotypically similar organisms." Thin-layer chromatography of e...Continue Reading

References

Apr 11, 1992·Oral Microbiology and Immunology·M O WasfyW A Falkler
Dec 31, 2010·Biotechnology and Bioengineering·Michael BraunDirk Weuster-Botz
Nov 22, 2005·Journal of Lipid Research·Jason M RidlonPhillip B Hylemon
Aug 12, 2014·The Journal of Membrane Biology·Jian-Shan Cai, Jin-Hong Chen
Nov 27, 2016·Applied Microbiology and Biotechnology·Christopher StaleyMichael J Sadowsky
Jan 1, 1993·Journal of Basic Microbiology·H GrohC Hörhold-Schubert
Jul 21, 2015·Nature Chemical Biology·A Sloan Devlin, Michael A Fischbach

Related Concepts

Arginine hydrochloride
Butyribacterium
HSD17B6
3-Hydroxysteroid Dehydrogenases
Substrate Specificity
Cell-Free System
Bile Salts
Hydrogen-Ion Concentration
Hydroxysteroid Dehydrogenases

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