Bile Salt Hydrolase (Bsh) Activity Screening of Lactobacilli: In Vitro Selection of Indigenous Lactobacillus Strains with Potential Bile Salt Hydrolysing and Cholesterol-Lowering Ability

Probiotics and Antimicrobial Proteins
Rajesh KumarVirender Kumar Batish

Abstract

The bile salt hydrolase (Bsh) activity of probiotic bacterium residing in gastrointestinal tract has often being associated with its cholesterol-lowering effects. Hence, Bsh activity was explored in this study as the criterion for the selection of most potential Bsh-active and cholesterol-lowering indigenous Lactobacillus strains. Forty lactobacilli were adjudged Bsh active after a preliminary screening of 102 lactobacilli and occurrence of Bsh activity correlated well with their natural habitats. Of the 40 shortlisted lactobacilli, fifteen putative Lactobacillus strains were selected and further tested for their comparative Bsh activity. In the end, indigenous Lactobacillus plantarum strains Lp91 and Lp21 were emerged as the promising Bsh-active lactobacilli with their substrate preference inclined more towards glycocholate than other bile acid amino conjugates. In addition, strains Lp91 and Lp21 also exhibited significantly high bile salt deconjugation, cholesterol assimilation and cholesterol co-precipitation ability in vitro. In conclusion, indigenous L. plantarum strains Lp91 and Lp21 may be the promising candidate probiotics to elucidate the ecological significance of probiotic Bsh activity in vivo.

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Citations

Jan 1, 2014·Biotechnology Letters·Xiang-Chao GuTong-Cun Zhang
Apr 1, 2014·Genes & Nutrition·Raj Kumar DuarySunita Grover
Mar 13, 2014·Journal of Bioscience and Bioengineering·Marimuthu Anandharaj, Balayogan Sivasankari
Nov 15, 2015·Probiotics and Antimicrobial Proteins·R González-VázquezY Rivera-Espinoza
Mar 7, 2009·BMC Microbiology·Orla O'SullivanTom Beresford
Sep 13, 2014·Archives of Microbiology·Ashok Kumar YadavVirender Kumar Batish
Feb 7, 2017·Standards in Genomic Sciences·Mangesh V SuryavanshiYogesh S Shouche
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Dec 1, 2013·Probiotics and Antimicrobial Proteins·Guiomar Melgar-LalanneHumberto Hernández-Sánchez
Feb 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Matthew H FoleyCasey M Theriot
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May 1, 2021·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Saranya WongrattanapipatMassalin Nakphaichit
Aug 8, 2021·International Journal of Molecular Sciences·Margaret VourakisGuy Rousseau

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